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#alpha#_(1)-肾上腺素受体亚型介导细胞内游离Ca~(2+)增高的信号转导途径

摘要

探讨了#alpha#_(1),#alpha#_(1b),#alpha#_(1d)三种亚型肾上腺素受体(AR)激动时细胞内Ca~(2+)浓度升高的信号转导途径。在稳定表达三亚型#alpha#_(1)-AR的HEK293细胞系中,用fura-2方法描记细胞内Ca~(2+)信号强弱的变化。

关键词： α_(1)-肾上腺素受体; 受体亚型; G蛋白; 磷脂酶C; 蛋白激酶C; 环-磷酸腺苷; 酪氨酸激酶; fura-2

Pathways of signal transduction of intracellular Ca~(2+) increase mediated by three subtypes of #alpha#_(1)-adrenoceptor in HEK293 cells

Abstract

In HEK293 cell lines in which #alpha#_(1a)-, #alpha#_(1b)- or #alpha#_(1d)-adrenoceptor subtypes were stably expressed, fluorescence intensities due to traces of Ca~(2+) were investigated using fura-2/AM with the aim of exploring signal transduction pathways of intracellular Ca~(2+) increase mediated by these receptor subtypes.

Key words: #alpha#_(1)-adrenoceptors;Receptor subtype;G protein;Phospholipase C;Protein kinase C;Cyclic AMP;Tyrosine kinase;Fura-2

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引用本文：

. #alpha#_(1)-肾上腺素受体亚型介导细胞内游离Ca~(2+)增高的信号转导途径[J]. 生理学报 1998; 50 (3): .

. Pathways of signal transduction of intracellular Ca~(2+) increase mediated by three subtypes of #alpha#_(1)-adrenoceptor in HEK293 cells. Acta Physiol Sin 1998; 50 (3): (in Chinese with English abstract).