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过量表达Wnt-1基因诱导P19细胞的神经分化

摘要

利用克隆到的Wnt-1基因转染P19细胞(Wnt-1/P19),可使细胞不经视黄酸(RA)诱导,自发向神经细胞方向分化。早期神经分化关键基因MASH-1在Wnt-1/P19细胞的神经分化过程中的表达,晚于RA诱导引起的P19细胞的分化过程。

关键词： Wnt-1; 反转录PCR; P19细胞; 过量表达; 神经分化; MASH－1

Neural differentation of Wnt-1 overexpression P19 cells

Abstract

Wnt-1 gene plays important roles during the development of the mouse central neural system, and shown a transient expression during neuronal differentiation of P19 EC cells. We transfected Wnt-1 gene into P19 cells and found the Wnt-1 transfected cells could differentiate into neurons without the induction of RA. However, MASH-1, a crucial gene for the early neural differentiation, was expressed later than P19 cells induced by RA.

Key words: Wnt-1;Reverse transcriptional PCR;P19 cells;Overexpression;Neuronal differentiation;MASH-1

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引用本文：

. 过量表达Wnt-1基因诱导P19细胞的神经分化[J]. 生理学报 1998; 50 (3): .

. Neural differentation of Wnt-1 overexpression P19 cells. Acta Physiol Sin 1998; 50 (3): (in Chinese with English abstract).