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红光抑制绿豆下胚轴伸长时富含羟脯氨酸蛋白质的含量变化

龙程, 张志良, 沈曾佑, 颜季琼

华东师范大学生物系.上海 200062

摘要

红光能增加细胞壁的Hyp含量和细胞各部分的Hyp/Pro,绿豆下胚轴切段伸长被红光抑制的程度与两者呈正相关。环己酰亚胺和放线菌素D明显降低细胞壁的Hyp含量,提高WSFC和CWRSE中的Hyp/Pro,抑制切段伸长。

关键词： 红光; 绿豆下胚轴; 伸长; 羟脯氨酸; 羟脯比

Changes in content of hydroxyproline-rich glycoprotein in red-light-ionhibited elongation growth of Phaseolus radiatus L. hypocotyls

Long Cheng, Zhang Zhiliang, Shen Zengyou, Yan Jiqiong

Department of Biology,East China Normal University.Shanghai 200062

Abstract

The labeling of various fractions of cell wall with ~(3)H-proline (Pro) and ~(3)H-hydroxyproline (Hyp) was measured radiochromatographyically and the hydroxyproline content was analyzed photometrically in the cell wall of Phaseolus radiatus L. hypocotyls. The hydroxylation of proline occurred in the water-soluble fraction of cytoplasm (WSFC), sodium dodecyl sulfate extract of cell wall (SECW) and cell wall residue after sodium dodecyl sulfate extraction (CWRSE), but to a larger extent in WSFC and SECW than in CWRSE. Red light increased the Hyp content in cell wall and the Hyp/Pro ratio of all three fractions, indicating that they were correlated with the inhibition of hypocotyl elongation by red light. Although ethylene glycol-bis (2-aminoethyl ether)-N, N, N^, N^-tetraacetic acid (EGTA), verapamil, La~(3+) and A_(23187) could eliminate the inhibition of hypocotyl elongation growth by red light, there was no significant effect on the Hyp content in cell wall or the degree of hydroxylation of proline in the three fractions after treatment with them.

Key words: Red light;Phaseolus radiatus;Hypocotyl;Elongation;Hydroxyproline

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引用本文：

龙程, 张志良, 沈曾佑, 颜季琼. 红光抑制绿豆下胚轴伸长时富含羟脯氨酸蛋白质的含量变化[J]. 生理学报 1998; 50 (2): .

Long Cheng, Zhang Zhiliang, Shen Zengyou, Yan Jiqiong. Changes in content of hydroxyproline-rich glycoprotein in red-light-ionhibited elongation growth of Phaseolus radiatus L. hypocotyls. Acta Physiol Sin 1998; 50 (2): (in Chinese with English abstract).