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血管紧张素II诱导培养的成年大鼠心肌细胞凋亡

摘要

研究血管紧张素II(AngII)诱导培养的成年大鼠心肌细胞凋亡。酶灌流消化法分离培养ARVMs,不同处理后,光镜观察形态改变,琼脂糖凝胶电泳定性分析DNA降解程度。结果发现培养的ARVMs经AngII10#mu#mol/L处理48h后,大部分细胞变圆,胞浆浓缩;电泳显示核酸断裂片段“梯形”结构,上述改变在72h更为明显。上述作用可被氯沙坦、维拉帕米和staurosporine所取消。这表明AngII由AT_(1)受体介导诱导培养的ARVMs凋亡,细胞内钙升高和PKC激活起重要作用。

关键词： 血管紧张素Ⅱ; 凋亡; 氯沙坦; 心肌细胞; 大鼠

Angiotensin II-induced apoptosis in cultured adult rat ventricular myocytes

Abstract

Angiotensin II (AngII)-induced apoptosis was demonstrated for the first time in cultured adult rat ventricular myocytes (ARVMs) isolated by retrograde heart perfusion with Krebs-Henseleit bicarbonate (KHB) buffer containing collagenase and hyaluronidase. ARVMs incubated with 10#mu#mol/L Ang II for 48h showed morphological features of apoptosis (cellular shrinkage, condensation of cytoplasm) and a characteristic "ladder" of DNA bands representing integer multiples of the internucleosomal DNA length about 180-200bp, which became more evident with further incubation up to 72h. With shorter incubation time (≤24h) or at a lower Ang II concentration (<10#mu#mol/L), such changes failed to occur.

Key words: Angiotensin Ⅱ;Apoptosis;Losartan;Cardiomyocyte;Rat

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引用本文：

. 血管紧张素II诱导培养的成年大鼠心肌细胞凋亡[J]. 生理学报 1998; 50 (4): .

. Angiotensin II-induced apoptosis in cultured adult rat ventricular myocytes. Acta Physiol Sin 1998; 50 (4): (in Chinese with English abstract).