真菌诱导子对新疆紫草悬浮培养细胞的生长和紫草素合成的影响
摘要
4种真菌诱导子诱导新疆紫草悬浮培养细胞对细胞紫草素的合成均有促进作用,其中以黑曲霉诱导子效果最高,而且促进细胞紫草素的外排,约30%的紫草素存在于培养液中;诱导促使细胞苯丙氨酸解氨酶活性显著提高,培养液中紫草素的含量变化与苯丙氨酸解氨酶活性变化呈正相关性。
Effects of fungal elicitors on the cell growth and the shikonin biosynthesis in Arnebia euchroma cells in suspension culture
Abstract
The shikonin biosynthesis in Arnebia euchroma cells in suspension culture was enhanced by treating with four kinds of fungal elicitors separately, among them the Aspergillus niger elicitor had the highest inducing efficiency, and led to the secretion of secondary products, such that more than 30% of the shikonin produced was found in the medium. The elicitation depended on the types and concentration of the elicitor, and the physiological status of cells in suspension. The optimum concentration of A. niger elicitor was 160#mu#g/nl culture medium. The best resonse of A. euchroma cells to elicitor treatment was in the late exponential growth phase and the early stationary phase. After adding elicitor, both PAL activity of A. euchroma cells and the shikonin content in the medium were remarkably increased, showing positive relationship between them.
Key words: Fungal elicitor;Amebia euchoma;Cell suspension culture;Shikonin
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引用本文:
. 真菌诱导子对新疆紫草悬浮培养细胞的生长和紫草素合成的影响[J]. 生理学报 1998; 50 (1): .
. Effects of fungal elicitors on the cell growth and the shikonin biosynthesis in Arnebia euchroma cells in suspension culture. Acta Physiol Sin 1998; 50 (1): (in Chinese with English abstract).