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水稻中 p5 cs基因的存在及其在高脯氨酸变异系中的作用

摘要

用来自豇豆根瘤的脯氨酸合成酶△^-吡咯啉-5-羧酸合成酶cDNA作探针进行杂交分析,结果表明水稻中存在豇豆根瘤 p5 cs基因的同源序列,它在盐胁迫条件下转录水平提高。水稻高脯氨酸变异系杂交后代株系的脯氨酸含量和耐盐性均明显高于原始型。应用水稻高脯氨酸变异系为材料进行的试验,结果显示高脯氨酸特性和高耐盐性与此基因的存在及其转录水平的提高有相关性。

关键词： 脯氨酸; △’-吡咯啉-5-羧酸合成酶基因; 耐盐性; 水稻

The presence of p5 cs gene in rice and its function in proline-overproducing rice plant lines

Abstract

Hybridization analysis was conducted using proline synthase △^-pyrroline-5-carboxylate synthetase (P5CS) cDNA from root nodule of cowpea as probe. The results indicated the presence of the homologue sequence of p5cs gene of cowpea nodule in rice plant. Its transcription level increased under salt stress. The poline content and salt tolerance of the filial generation (F_(9)) of proline-overproducing rice plant lines were markedly higher than those of the original type. Using these proline-overproducing rice plant lines as experimental materials to conduct hybridization analysis, it has been shown that the high proline content characteristics and increased salt tolerance are correlated to the presence of this gene and its increased transcription.

Key words: Proline;#DELTA#’-pyrroline-5-carboxylate synthetase gene;Salt tolerance;Rice

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引用本文：

. 水稻中 p5 cs基因的存在及其在高脯氨酸变异系中的作用[J]. 生理学报 1998; 50 (1): .

. The presence of p5 cs gene in rice and its function in proline-overproducing rice plant lines. Acta Physiol Sin 1998; 50 (1): (in Chinese with English abstract).