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低温锻炼对水稻幼苗叶片中Rubisco的影响

赵军, 王以柔, 李美茹, 刘鸿先, 缪有刚, 李立人

中科院华南植物研究所. 广东, 广州 510650；中科院上海植物生理研究所. 上海 200032

摘要

低温锻炼能提高水稻幼苗的抗冷力, 低温锻炼虽不能明显提高Rubisco活性，却提高了冷胁条件下Rubisco的稳定性和增强了胁迫后正常生长条件下其活性的恢复能力。分别用火箭免疫电泳分析Rubisco蛋白和SDS-PAGE分析大、小亚基量表明：低温锻炼未提高Rubisco蛋白的合成能力，但增加了大、小亚基的合成量。经锻炼、冷胁迫以及恢复后Lsu/Ssu比值的变化主要是由于小亚基对温度变化更敏感所致。Rubisco酶特性分析表明, 低温锻炼有减少水稻幼苗Rubisco表面的SH数, 并提高Rubisco蛋白在高、低温下的稳定性。

关键词： 低温锻炼; 水稻幼苗; 抗冷性; 核酮糖二磷酸羧化酶

The effects of cold-hardening on Rubisco in rice seedling leaves

Zhao Jun, Wang Yirou, Li Meiru, Liu Hongxian, Miao Yougang, Li Liren

South China Institute of Botany, The Chinese Academy of Sciences. Guangzhou 510650, Guangdong； China

Abstract

The changes in activity, protein content and character of Rubisco in leaves caused by cold-hardening and chilling stress were studied. Theincrease of cold tolerance by cold-hardening in rice seedlings is associated with the change in Rubisco character.

Key words: Cold hardening;Rice seedlings;Cold resistant;Rubisco

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引用本文：

赵军, 王以柔, 李美茹, 刘鸿先, 缪有刚, 李立人. 低温锻炼对水稻幼苗叶片中Rubisco的影响[J]. 生理学报 1997; 49 (2): .

Zhao Jun, Wang Yirou, Li Meiru, Liu Hongxian, Miao Yougang, Li Liren. The effects of cold-hardening on Rubisco in rice seedling leaves. Acta Physiol Sin 1997; 49 (2): (in Chinese with English abstract).