C_(6)神经胶质瘤细胞的电压依赖性钾电流
何淑舫, 谢重
中科院上海脑研究所.上海 200031
摘要
用全细胞膜片箝方法对大鼠传代培养C_(6)神经胶质瘤细胞(C_(6)细胞)的电压依赖性K~(+)电流进行了研究。实验共记录了97个细胞,膜电位均值(SD)为-47.5±9.4mV。有7%的C_(6)细胞没有外向K~(+)电流。具有外向K~(+)电流的细胞,维持电压在-70mV时K~(+)电流的激活阈值为-36.5±5.9mV。通过对尾电流的分析其逆转电位(SD)为-84.6±4.1mV,接近于K~(+)平衡电位。在记录的细胞中所含K~(+)电流的成分不同,约占总数60%的细胞,其K~(+)电流包含呈暂态的快成分和呈稳态的慢成分。约占总数33%的细胞只具有慢成分。
关键词: C_(6)神经胶质瘤细胞; K-+电流; 全细胞膜片箝记最
Voltage-dependent potassium currents in rat C_(6) glioma cells
He Shufang, Xie Zhong
Shanghai Brain Research Institute, Chinese Academy of Sciences. Shanghai 200031
Abstract
In the present study, the properties of voltage-dependent K~(+) currents in cultured rat C_(6) glioma cells were investigated with the whole cell patch-clamp recording technique. A total of 97 cells were recorded. The mean value of the membrane potential was -47.5±9.4 mV. Seven percent of the reocrded C_(6) glioma cells did not show any outward potassium currents. The rest had outward K~(+) currents which could be activated at -36.5±5.9 mV from a holding potential of -70 mV. Ananalysis of tail currents, showed the mean value of the reversal potential was -84.6±4.1 mV, close to the K~(+) equilibrium potential. The K~(+) currents of 60% of the recorded C_(6) cells consisted of both a fast and a slow component, while those of the 33% only with a slow component.
Key words: C_(6) glioma cells;K-+ current;Whole-cell petch-clamp recording
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引用本文:
何淑舫, 谢重. C_(6)神经胶质瘤细胞的电压依赖性钾电流[J]. 生理学报 1997; 49 (1): .
He Shufang, Xie Zhong. Voltage-dependent potassium currents in rat C_(6) glioma cells. Acta Physiol Sin 1997; 49 (1): (in Chinese with English abstract).