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用显微光度图像分析系统研究谷子胚性细胞系的GDH细胞类型分布

董晋江, 夏镇澳

中科院上海植物生理研究所.上海 200032

摘要

由显微光度图像分析与细胞化学技术对不同实验材料和不同培养时期谷子胚性细胞系的谷氨酸脱氢酶(GDH)细胞类型分布表明:从单细胞群体水平(细胞类型分布)研究胚性细胞系可以比较准确地了解其所处的生理状态;胚性细胞系的细胞类型分布可以作为其是否适于原生质体培养的指标之一;细胞类型分布也适用于组织培养和原生质体培养一些问题的研究。

关键词： 谷子; 胚性细胞系; 显微光度图像分析; 谷氨酸脱氢酶(GDH); 细胞类型分布

Studies on the cell type compositions of embryogenic callus of foxtail millet by using the micro-optic density image analysis system

Dong Jinjiang, Xia Zhenao

Shanghai Institute of Plant Physiology, The Chinese Academy of Sciences. Shanghai 200032

Abstract

The physiological state of embryogenic cell lines is one of the most unstable factors, which often lowers the efficiency of protoplast culture system of cereal crops. In this report, preliminary results of studies on the glutamate dehy-drogenase (GDH) cell type compositions by using cytochemistry and microoptic density image analysis system were presented. The results showed that it was more accurate to assess the physiological states of embryogenic cell lines by using single cell population; the GDH cell type compositions of embryogenic cell lines could be used as a measure of the suitability of the cell lines for protoplast culture; the GDH cell type compositions could also be applied to the study of protoplast culture and tissue culture.

Key words: Foxtail millet;Embryogenic cell line;Micro-optic density image analysis;Glutamate dehydngenase;Cell type compositions

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引用本文：

董晋江, 夏镇澳. 用显微光度图像分析系统研究谷子胚性细胞系的GDH细胞类型分布[J]. 生理学报 1996; 48 (4): .

Dong Jinjiang, Xia Zhenao. Studies on the cell type compositions of embryogenic callus of foxtail millet by using the micro-optic density image analysis system. Acta Physiol Sin 1996; 48 (4): (in Chinese with English abstract).