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抗atrazine转基因大豆的抗性遗传及某些生理、农艺性状

岳绍先, 傅骏骅, 李连城, 苑红丽

中国农业科学院作物育种栽培研究所.北京 100081

摘要

抗atrazine基因导入大豆植株后,其F_(1)代植株对atrazine的抗性性状均与其母本性状一致,即该性状为母系遗传,为细胞质基因,位于叶绿体DNA上。通过导入抗性基因株与未导入抗性基因的对照株的光合速率测定、成分分析及农艺性状(株高、百粒重、单株产量)测定表明,两类植株间无明显差异。在喷施atrazine情况下,转基因株能表现出抗性。

关键词： 大豆; 阿特拉津; 转基因植株

The resistance inheritance and some physiological and agronomic traits of transgenic soybean plants with atrazine-resistance gene

Yue Shaoxian, Fu Junhua, Li Liancheng, Yuan Hongli

Institute of Crop Breeding & Cultivation, The Chinese Academy of Agricultural Sciences. Beijing 100081

Abstract

A comparative study of the mode of resistance inheritance and some physiological and agronomic traits has been conducted on the control plants and the transgenic plants from soybean line 83117 to which an atrazine-resistance gene was transferred. By examining the response of leaves to atrazine spray, molecular hybridization with a probe, assaying with flurescence induction kinetics and by examining the response of plants to atrazine spray in field, it has been proved that the resistance gene transferred into soybean plants has been not only expressed in F_(1) generation, but also transmitted to the progenies (7th generation in 1993). The experiment of reciprocal crossing (transgenic plant×control plant; control plant×transgenic plant) has proved that, the trait involved in the resistance to atrazine of F_(1) plant is the same as that of its mother plant, implying that this trait is inherited maternally, so this gene is a cytoplasmic one, located in the chloroplast. The results of photosynthesis measurements, seed composition analysis and examination of agronomic traits (plant height, 100 grain weight and yield per plant) indicated that there was no significant difference between the transgenic plants and ghese of the original line, so the resistance gene had no adverse effect on these traits. When sprayed with atrazine, the transgenic plants showed resistance to atrazine.

Key words: Soybean;Atrazine;Transgenic plant

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引用本文：

岳绍先, 傅骏骅, 李连城, 苑红丽. 抗atrazine转基因大豆的抗性遗传及某些生理、农艺性状[J]. 生理学报 1996; 48 (4): .

Yue Shaoxian, Fu Junhua, Li Liancheng, Yuan Hongli. The resistance inheritance and some physiological and agronomic traits of transgenic soybean plants with atrazine-resistance gene. Acta Physiol Sin 1996; 48 (4): (in Chinese with English abstract).