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水稻蜡质基因5^端上游顺序中的核蛋白结合区

陈丽, 王宗阳, 张景六, 蔡秀玲, 洪孟民

中科院上海植物生理研究所.上海 200032

摘要

通过wx基因转录起始点上游2 120 bp长的DNA序列的测定,用不同的限制性内切酶对此片段进行消化,获得15个大小不同的DNA片段并构建成不同的亚克隆。这些片段经~(32)P标记后分别做探针同水稻不同组织来源的核蛋白进行凝胶滞后实验和特异的竞争实验,表明HinfIa-2、HinfIc-1、HinfIc-2、HinfId、RsaIa和RsaIe (RsaIa片段与HinfIa、HinfIc片段重叠)片段能与从未成熟的水稻种子中抽提的核蛋白结合。

关键词： DNA结合蛋白; 水稻; 蜡质基因

Nuclear protein binding sites in 5^-upstream region of the rice waxy gene

Chen Li, Wang Zongyang, Zhang Jingliu, Cai Xiuling, Hong Mengmin

Shanghai Institute of Plant Physiology, The Chinese Academy of Sciences. Shanghai 200032

Abstract

A 5^-upstream region (from-2 120 bp to transcription start site of this gene) was sequenced, fifteen small fragments were obtained and subcloned from the 2 120 bp fragment by different restriction enzymes. These fragments were labelled with ~(32)P and used as probes in gel retardation and cognate DNA competition assays. The results showed that HinfIa-2, HinfIc-1, HinfIc-2, HinfId, RsaIa (which overlaps with HinfIa and HinfIc) and RsaIe could interact with nuclear protein factors. In addition, HinfIa-2, HinfIc-1, HinfIc-2, HinfId and RsaIe DNA fragments could also interact with the proteins extracted from rice pollen. All these results demonstrate that at least five nuclear protein binding sites are located in the 5^ upstream region of rice waxy gene.

Key words: DNA-binding protein;Rice;Waxy gene

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引用本文：

陈丽, 王宗阳, 张景六, 蔡秀玲, 洪孟民. 水稻蜡质基因5^端上游顺序中的核蛋白结合区[J]. 生理学报 1996; 48 (4): .

Chen Li, Wang Zongyang, Zhang Jingliu, Cai Xiuling, Hong Mengmin. Nuclear protein binding sites in 5^-upstream region of the rice waxy gene. Acta Physiol Sin 1996; 48 (4): (in Chinese with English abstract).