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Na_(2)SO_(3)对不同状态CF_(1)-ATPase活力的促进作用

何向东, 杜子云, 李有则

中科院上海植物生理研究所.上海 200032

摘要

Na_(2)SO_(3)对CF_(1)-ATPase活力的促进作用与酶所处状态有关。CF_(0)降低CF_(1)对Na_(2)SO_(3)的亲和力和Na_(2)SO_(3)促进的最大反应速率。在Na_(2)SO_(3)作用下,膜上CF_(1)-ATPase的活化能高于游离的。膜上和游离CF_(1)-ATPase的#gamma#亚基上二硫键的还原可以提高NA_(2)SO_(3)对酶活力的促进作用。Na_(2)SO_(3)对甲醇活化的CF_(1)-ATPase活力的促进作用只有在甲醇活化的亚适浓度下才能充分表现出来。Na_(2)SO_(3)对Mg~(2+)抑制的解除作用因CF_(1)-ATPase处于不同活化状态而不同。

关键词： 叶绿体H~(+)－ATPase; 热活化; 热/DTT活化; 甲醇活化; Na_(2)SO_(3)

Study on the stimulative effect of sulfite on the activity of CF_(1)-ATPase in different states

He Xiangdong, Du Ziyun, Li Youze

Shanghai Institute of Plant Physiology,The Chinese Academy of Sciences.Shanghai 200032

Abstract

The effects of sulfite on CF_(1)-ATPase in different methods were studied. Sulfite stimulate stimulated the activity of heat/DTT-activated soluble and thylakoid membrane-bound CF_(1)-ATPase activity was weaker. The affinity of heat/DTT-activated membrane-bound CF_(1)-ATPase for sulfite and the maximal activity of heat/DTT-activated membrane-bound CF_(1)-ATPase stimulated by sulfite were lower than those of soluble CF_(1)-ATPase. In the presence of sulfite, the activation energy of thylakoid membrane-bound CF_(1)-ATPase was higher than that of soluble CF_(1)-ATPase. These results indicate that the catalytic function of CF_(1) is controlled by membrane-bound CF_(0). The reduction of disulfide bond in γ subunit could further increase the stimulative effect of sulfite on heat- or methanol-activated soluble and thylakoid-membrane-bound CF_(1)-ATPase. The stimulative effect of sulfite on the methanol-activated soluble and thylakoid membrane-bound CF_(1)-ATPase was dependent on the methanol concentration in the reaction medium. Its stimulative effect was more pronounced at sub-optimal methanol concentrations than at the optimum concentration. The relieving effect of sulfite on the inhibiton of the methanol-activated CF_(1)-ATPase activity by Mg~(2+) was different from that of the heat/DTT-activated CF_(1)-ATPase. Why sulfite has different effects in relieving the inhibition of the activity of differently activated CF_(1)-ATPase by Mg~(2+) needs further study.

Key words: Chloropldet H~(+)-ATPase;Heat activation;Heat/DTT activation;Methanol activation;Sulfite

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引用本文：

何向东, 杜子云, 李有则. Na_(2)SO_(3)对不同状态CF_(1)-ATPase活力的促进作用[J]. 生理学报 1996; 48 (3): .

He Xiangdong, Du Ziyun, Li Youze. Study on the stimulative effect of sulfite on the activity of CF_(1)-ATPase in different states. Acta Physiol Sin 1996; 48 (3): (in Chinese with English abstract).