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扁豆几丁酶的特性和诱导

夏玉先, 裴炎, 李先碧, 张林

西南农业大学生物技术研究中心.重庆 630716

摘要

采用再生几丁质亲和层析和两性电解质等电聚焦电泳,纯化了扁豆荚几丁酶,其分子量30kD,等电点为9.1,主要呈内切酶活性。扁豆荚中不同组织几丁酶比活力有很大差异。在豆荚发育过程中,其酶活性变化呈单峰曲线,而比活力则持续上升,表明扁豆几丁酶活性变化与发育相关。经HgCl_(2)处理后,扁豆荚壳和种子的几丁酶活性均明显提高。在扁豆荚发育的不同阶段,几丁酶的诱导特性也有明显差异,幼嫩豆荚几丁酶诱导活性的增加更为明显。

关键词： 扁豆; 几丁酶; 纯化; 发育; 诱导

Induction and characterization of chitinase in hyscinth pea pods

Xia Yuxian, Pei Yan, Li Xianbi, Zhang Lin

Southwest Agricultural University,Biotechanology Research Center.Chongqing 630716

Abstract

Chitinase (EC 3.2.1.14) was purified from the hulls of hyacinth pea pods by affinity chromatography on a column of regenerated chitin and by isoelectric focusing. The purified chitinase gave a single protein band on sodium dodecyl sulfate polyacrylamide gel electrophoresis with a molecular weight of 30 kD. The enzyme was proved to be endochitinase and the isoelectric point (pI) was pH 9.1. The specific activity of the chitinase in the hulls of hyacinth pea pods was about 17 times as high as that in the cotyledons and 11 times that in the embryo. The specific activity of chitinase in the hull increased with development and maturation of the pods. The time course of the change of chitinase activity had a single peak on the 13th day after anthesis. The results indicate that chitinase activity is in relationship with development. The chitinase activity in the hulls and in the seeds of the pods treated with HgCl_(2) was higher than in those of untreated ones. The chitinase activity increase induced by HgCl_(2) was larger in immature pods than in mature ones.

Key words: Hyacinth pea;Chitinase;Purification;Development;Induction

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引用本文：

夏玉先, 裴炎, 李先碧, 张林. 扁豆几丁酶的特性和诱导[J]. 生理学报 1996; 48 (3): .

Xia Yuxian, Pei Yan, Li Xianbi, Zhang Lin. Induction and characterization of chitinase in hyscinth pea pods. Acta Physiol Sin 1996; 48 (3): (in Chinese with English abstract).