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干旱和盐胁迫诱导甜菜叶中的甜菜碱醛脱氢酶的积累

梁峥, 骆爱玲, 赵原, 汤岚

中科院植物研究所.北京100044

摘要

应用双向免疫扩散方法测定表明,甜菜叶片的甜菜碱醛脱氢酶能与菠菜的甜菜碱醛脱氢酶抗体发生交叉反应。渗透势－0.65-－2.6MPa的甘露醇溶液或200-300mmol/L的NaC1溶液,诱导甜菜叶片甜菜碱醛脱氢酶积累明显增加。

关键词： 干旱胁迫; 盐胁迫; 甜菜碱醛脱氢酶; 免疫方法; 甜菜

Accumulation of betaine aldehyde dehydrogenase induced by drought and salt stress in sugar beet leaves

Liang Zheng, Luo Ailing, Zhao Yuan, Tang Lan

Institute of Botany,The Chinese Academy of Sciences.Beijing 100044

Abstract

The antiserum formed against the BADH from spinach leaves were allowed to cross-react with the crude BADH preparation from sugar beet leaves.The accumulation of BADH in the leaves or leaf discs was detected by immunodiffusion method.When sugar beet seedlings were subjected to a salt stress of 300 mmol/L,the accumulation of BADH was about 2 folds higher than that in control leaves.The BADH in the sugar beet leaf discs floated on a solution of NaC1 200 mmol/L for 13 h was also 2-4 folds higher than that in the control discs. When leaf discs were subjected to drought stress of -0.65-2.6 MPa for 18h,the BADH induced in the leaf discs increased about 2-4 folds.These results pointed to the importment function of BADH in the adaptation to drought and salt stress.

Key words: Drought stress;Salt stress;Betaine aldehyde dehydrogenase;Immunological method;Sugarbeet

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引用本文：

梁峥, 骆爱玲, 赵原, 汤岚. 干旱和盐胁迫诱导甜菜叶中的甜菜碱醛脱氢酶的积累[J]. 生理学报 1996; 48 (2): .

Liang Zheng, Luo Ailing, Zhao Yuan, Tang Lan. Accumulation of betaine aldehyde dehydrogenase induced by drought and salt stress in sugar beet leaves. Acta Physiol Sin 1996; 48 (2): (in Chinese with English abstract).