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小麦根质膜原位膜微囊与翻转膜微囊的氧化还原特性比较

邱全胜, 梁厚果, 焦新之, 倪晋山

兰州大学植物生理研究室.甘肃,兰州 730001；中科院上海植物生理研究所.上海 200032

摘要

用二相法和不连续蔗糖梯度离心分别制得小麦根质膜的原位膜微囊和翻转膜微囊。两者比较可知:质膜内外两侧均表现出较高的氧化还原活性;膜内侧的NAD(P)H氧化和Fe(CN)~(3-)_(6)还原速率高于外侧。质膜内外两侧都能还原EDTA-Fe~(3+),但外侧的还原活性高于内侧。质膜内外两侧均有O_(2)吸收,同时都可被SHAM刺激,被KCN抑制。质膜内侧和外侧都可产生O~(-)_(2),最适pH值为6.0;即可被SHAM刺激,也可被SOD、过氧化氢酶和KCN抑制。

关键词： 质膜; 原位膜微囊; 翻转膜微囊; 氧化还原系统; 产生; 小麦根

Comparison of the redox properties of right-side out and inside out plasmalemma vesicles purified from wheat roots

Qiu Quansheng, Liang Hougou, Jiao Xinzhi, Ni Jinshan

Laboratory of Physiology,Lanzhou University.Lanzhou 730001,Gausu；China

Abstract

Plasmalemma vesicles were purified from 8-day-old wheat (Triticum asetivum var.Longchun No.10) roots by discontinuous sucrose gradient centrifugation (SG) and aqueous two-phase partitioning (PP).Both IO and RO had high redox activities. The rates of NAD(P)H oxidation and Fe(CN)~(3)-_(6) reduction by IO was higher than that by RO while the EDTA-Fe~(3+) reduction of IO was lower than that RO.SHAM-stimulated NADH oxidation by IO was signifiicant higher than that by RO;But SHAM-stimulated O_(2) uptake by IO was only slightly higher than that by RO. And SHAM-stimulated NADH oxdation and O_(2) uptake by both IO and RO were completely inhibited by KCN.Both IO and RO generated O~(-)_(2) with an optimum pH value of 6.0.The O~(-)_(2) production by IO and RO was stimulated by SHAM and inhibited by SOD,catalase and KCN.

Key words: Plasmalemma;Right-side out vesicles;Inside out vesicles;Redox system;O_(2) generation;Wheatroots

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引用本文：

邱全胜, 梁厚果, 焦新之, 倪晋山. 小麦根质膜原位膜微囊与翻转膜微囊的氧化还原特性比较[J]. 生理学报 1996; 48 (2): .

Qiu Quansheng, Liang Hougou, Jiao Xinzhi, Ni Jinshan. Comparison of the redox properties of right-side out and inside out plasmalemma vesicles purified from wheat roots. Acta Physiol Sin 1996; 48 (2): (in Chinese with English abstract).