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TIBA、LaCl_(3)、Verapamil和TFP对菜豆下胚轴生长素诱导的导管分化和形成的效应

李焕秀, Battey N

四川农业大学.四川,雅安 625014；Reading 大学 Reading RG6 2AS

摘要

通过TIBA、LaCl_(3)、Verapamil和TFP对菜豆下胚轴导管分化和形成的调控作用,证明生长素、Ca~(2+)和CaM是木质部导管分化和形成所必需的。NAA能诱导菜豆下胚轴导管的分化;TIBA能抑制生长素类诱导的导管分化和形成;LaCl_(3)和TFP在导管分化诱导期(最初24h)具抑制作用;Verapamil与TIBA相似,在导管分化诱导期和导管形成期(伤口形成后72h之内)都有抑制作用。

关键词： 生长素; 菜豆; 导管分化和形成; Ca~(2+)通道拮抗剂; CaM拮抗剂

Effects of TIBA,LaCl_(3),verapamil and TFP on auxin-induced tracheary differentiation and formation of bean hypocotyls

Li Huanxiu, Battey N

Sichuan Agricultural University.Yaan 625014,Sichuan

Abstract

Ten-day old bean seedlings (Phaseolus vulgaris L.) were treated with several chemical substances in the way shown in Fig. 1. After 5 days^ treatment, bean hypocotyls were cut into upper and lower parts at wound and 2. 0 cm above and below the wound. The two parts were put into freshly boiled lactic acid solutions (80%) for 3 d. Newly formed tracheas between 8 original vascular bundles were counted under microscope(×50). The results were as follows:The optimum NAA concentration for the induction of trachea formation was 10~(-4)mol/L. The key time for induction was the first 24 h. TIBA inhibited auxin-induced trachea formation in 72 h after wound formation. The maximum inhibition by TIBA occurred in the first 24 h. LaCl_(3) had inhibitive action on trachea only when used in the first 24 h, and Verapamil did so in 72 h after wound formation although both were Ca~(2+) channel blockers. TFP, a CaM antagonist, inhibited trachea formation only when used in the first 24 h. It was suggested that auxin transport and the presence of CaM and Ca~(2+) were important for trachea formation in bean hypocotyls. Trachea formation may begin at 24-48 h after wound formation.After 72 h,auxin-induced trachea formation ended, after which inhibitors did not affect auxin-induced trachea formatiom.

Key words: Auxin;Bean;Trachea differentiation and formation;Ca~(2+)channel blocker;CaM antagonist

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引用本文：

李焕秀, Battey N. TIBA、LaCl_(3)、Verapamil和TFP对菜豆下胚轴生长素诱导的导管分化和形成的效应[J]. 生理学报 1996; 48 (1): .

Li Huanxiu, Battey N. Effects of TIBA,LaCl_(3),verapamil and TFP on auxin-induced tracheary differentiation and formation of bean hypocotyls. Acta Physiol Sin 1996; 48 (1): (in Chinese with English abstract).