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氧自由基对大鼠心肌#alpha#_(1)肾上腺素受体及其亚型的影响

宫海滨, 韩启德

北京医科大学第三医院血管医学研究所. 北京 100083

摘要

采用放射配体结合方法观察外源性氧自由基对大鼠心肌#alpha#_(1)肾上腺素受体(#alpha#_(1)受体)及其亚型#alpha#_(1A)与#alpha#_(1B)的影响, 表明氧自由基可直接作用于心肌膜, 引起#alpha#_(1)受体数量和亲和性下降, #alpha#_(1)受体亚型#alpha#_(1A)与#alpha#_(1B)之间的比例改变。

关键词： 心肌; 肾上腺素受体; 受体亚型; 氧自由基

Effects of oxygen free radicals on myocardial #alpha#_(1)-adrenoceptor and its subtypes in rat

Gong Haibin, Han Qide

Institute of Vascular Medicine, The Third Hospital, Beijing Medical University. Beijing 100083

Abstract

The effects of oxygen free radicals on myocardial #alpha#_(1)-adrenoceptor and its subtypes and #beta#-adrenoceptor were observed by the method of radioligand binding assay in rat. The experiments showed: (1)·OH and O~(.)_(2) reduced the B_(max) of #alpha#_(1)-adrenoceptors binding to~(125)IBE2254 by 55% and 36%, respectively. (2) The K_(1) values of #alpha#_(1)-adrenoceptors were increased by ·OH(by 171%), but not by O~(.)_(2) increased the ratio of #alpha#_(1A) vs #alpha#_(1B) subtypes. (4)#beta#-adrenoceptors were not changed by either ·OH or O~(.)_(2). (5)Oxygen free radical scavengers mannitol and superoxide dismutase were capable of preventing the changes of #alpha#_(1)-adrenoceptor and its subtypes induced by oxygen free radicals.

Key words: Free radicals;Heart;;

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引用本文：

宫海滨, 韩启德. 氧自由基对大鼠心肌#alpha#_(1)肾上腺素受体及其亚型的影响[J]. 生理学报 1996; 48 (1): .

Gong Haibin, Han Qide. Effects of oxygen free radicals on myocardial #alpha#_(1)-adrenoceptor and its subtypes in rat. Acta Physiol Sin 1996; 48 (1): (in Chinese with English abstract).