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低氧诱导兔血MDA和棘状红细胞增加及其被MPEG-SOD抑制

韩沾元, 陈铭, 吕及忍, 闻萍, 宋雪蕾, 吴奇英

中科院上海生理研究所. 上海 200031；上海医药工业研究所. 上海 200040；复旦大 学生物系. 上海

摘要

在低氧1,2,3,5和24h, 兔红细胞和血浆中丙二醛(MDA)明显增加。在低氧前由腹腔注 入维生素E(VE), 则明显抑制低氧诱导血浆MDA增加, 但对红细胞MDA增加的抑制无统计 学意义。低氧24h, 血液中棘状红细胞明显增加。然而在低氧前30min由股静脉注入单 甲氧基聚乙二醇-SOD, 低氧诱导红细胞MDA和血浆MDA增加以及棘状红细胞增多均被明 显抑制。

关键词： 低氧; 血液; 单甲氧基聚乙二醇－超氧化物歧化酶; 丙二醛; 超氧自由基

Hypoxia induced increase of MDA and echinocytes from erythrocytes in rabbit^s blood with special reference to inhibition of the increase by MPEG-SOD

Han Zhanyuan, Chen Ming, Lu Jiren, Wen Ping, Song Xuelei, Wu Qiying

Shanghai Institute of Physiology, Chinese Academy of Sciences. Shanghai 200031； China；Department of Biology, Fudan University. Shanghai

Abstract

When rabbits acutely exposed to hypobaric hypoxia MDA (malondialdehyde) in erythrocyte and plasma increased significantly during hypoxia periods of 1,2,3,5 and 24h. The increase of MDA in plasma could be prevented by vitamin E, but that in erythrocyte did not show significant change. The echinocytes from erythrocytes increased significantly after hypoxia for 24h (n=5, P<0.05vs. normoxia group). However, by injecting MPEG-SOD(monomethoxypolythlene glycol-SOD) intravenously 30 min before hypoxia, the increase of MDA (both of erythrocyte and plasma)and echinocytes(from erythrocytes)were both inhibited obviously during hypoxia for 24h(n=5, P<0.05, P<0.01 vs. hypoxia group).

Key words: Hypoxia;Blood;Monomethoxypolythlene glycol-SOD;Malondialdehyde;Superoxide

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引用本文：

韩沾元, 陈铭, 吕及忍, 闻萍, 宋雪蕾, 吴奇英. 低氧诱导兔血MDA和棘状红细胞增加及其被MPEG-SOD抑制[J]. 生理学报 1995; 47 (6): .

Han Zhanyuan, Chen Ming, Lu Jiren, Wen Ping, Song Xuelei, Wu Qiying. Hypoxia induced increase of MDA and echinocytes from erythrocytes in rabbit^s blood with special reference to inhibition of the increase by MPEG-SOD. Acta Physiol Sin 1995; 47 (6): (in Chinese with English abstract).