过刊浏览

肾上腺髓质素(13-52)降压机制的探讨

赵东, 田青, 赵云涛, 宫长宁, 韩启德, 张肇康, 汤健

北京医科大学心血管基础研究所. 北京 100083；海军总医院. 北京 100037

摘要

在整体和离体大鼠模型上观察研究了肾上腺髓质素(M-52)[AdM(13-52)]的降压机制, 发现Adm (M-52)的降压作用可被一氧化氮合酶(NOS)的竞争性拮抗剂L-N~(G)-硝基-精氨酸(LNNA)部分抑制; AdM(13-52)的舒血管作用依赖于血管内皮并可被LNNA抑制, 且具有 剂量效应关系, LNNA的这种效应可被L-氨基酸(L-Arg)逆转; 用亚甲蓝(MB)阻断血管内的 环-磷酸鸟苷酸(cGMP), 则导致Adm(13-52)的舒血管作用消失; 放免测定显示LNNA可以降 低血管内cGMP含量, 而AdM(13-52)则使后者含量增加, 这一现象在AdM(13-52)与LNNA合用时消失。图4表1参11

关键词： 肾上腺髓质素; 一氧化氮

A study on hypotensive mechanism of adrenomedullin (13-52)

Zhao Dong, Tian Qing, Zhao Yuntao, Gong Changning, Han Qide, Zhang Zhaokang, Tang Jian

Institute of Cardiovascular Research, Beijing Medical University. Beijing 100083；China

Abstract

In the present study the hypotensive mechanism of AdM (13--52) was investigated in rats, both in vitro and in vivo. It was found that the hypotensive effect of AdM (13--52) could be partially inhibited by L--N~(G)--nitro--arginine (LNNA), an inhibitor of nitric oxide synthase. The vasodilator effect of AdM (13--52) was dependent on vascular endothelium and inhibitor by LNNA in a dose--dependent manner. This LNNA induced inhibitory effect could be reversed with L--Arginine. In addition, the vasodilator effect of AdM (13--52) disappeared with methylene blue (MB), which blocked cGMP formation. Using radioimmunoassay it was shown that LNNA lowered, but AdM (13--52) elevated the vascular cGMP content, while vascular cGMP content was not altered by co--application of AdM (13--52) and LNNA. The above results suggest that the vasodilator effect of AdM (13--52) might be mediated by nitric oxide.

Key words: Adrenomedullin;Nitric oxide

收稿日期：　　录用日期：

通讯作者：　　E-mail:

引用本文：

赵东, 田青, 赵云涛, 宫长宁, 韩启德, 张肇康, 汤健. 肾上腺髓质素(13-52)降压机制的探讨[J]. 生理学报 1995; 47 (3): .

Zhao Dong, Tian Qing, Zhao Yuntao, Gong Changning, Han Qide, Zhang Zhaokang, Tang Jian. A study on hypotensive mechanism of adrenomedullin (13-52). Acta Physiol Sin 1995; 47 (3): (in Chinese with English abstract).