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糖皮质激素及其他甾体激素对大鼠下丘脑薄片释放精氨酸加压素的影响

刘秀, 王春安, 叶阿莉, 江萍, 陈宜张

第二军医大学生理教研室. 上海 200433；宜春卫生学校生理教研室. 江西, 宜春

摘要

大鼠下丘脑薄片经过90min的恢复之后, 在长达6h的孵育过程中能够相当稳定地释放精氨酸 加压素(AVP), 释放量为9.06±1.23pg/min; 皮质酮(B)在20min内可明显地抑制AVP的释放, 在10~(-7)-10~(-4)mol/L范围内呈剂量-效应关系; 在同一剂量(10~(-6)mol/L) 皮质醇、17#beta#-雌二酮和睾丸酮也可快速地抑制AVP的释放, 而相同剂量的 地地塞米松、醛固酮、孕酮、RU486和胆固醇却无此效应; RU486(10~(-7)-10~(3)mol/L)对AVP的释放没有影响, 但却能(10~(-6)10~(-3)mol/L)对AVP的释放没有影响, 但却能(10~(-6)mol/L部分地阻断的快速抑制效应。图4参14

关键词： 下丘脑薄片; 精氨酸加压素; 糖皮质激素; 非基因组机制

Effect of glucocorticoids and other steroids on arginine vasopressin release from rat hypothalamic slices

Liu Xiu, Wang Chunan, Ye Ali, Jiang Ping, Chen Yizhang

Department of Physiology, Second Military University. Shanghai 200433；China

Abstract

Rat hypothalamic slices sectioned with vibratone (containing paraventricular and supraoptic nuclei) were incubated in static microchamber and the Arginine vasopressin (AVP) released from the slices was measured by radioimmunoassay. The effect of glucocorticoids (GC) and other steroids on AVP release was investigated. The results were as 6 h (not includig the 90 min for recovery of slices). (2) Corticosterone (B) inhibited AVP release within 20 min in a dose-dependent manner from 10~(-7)to 10~(-4)mol/L. (3). Cortisol, 17 #beta#--estradiol, or testosterone (10~(-6)mol/L) also inhibited AVP release within 20 min, but dexamethasone, aldosterone, progesterone, RU 486, or cholesterol had no significant effect. (4)RU486 (10~(-5)--10~(-3)mol/L) could partially block the rapid inhibitory effect of corticosterone. These results suggested that GC might exert in situ a regulatory negative feedback action on the AVP release from hypothalamus of rat through a non--genomic rather than a genomic mechanism.

Key words: Hypothalamic slice;Aginine vasopressin;Glucocorticoids;Non-genomic

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引用本文：

刘秀, 王春安, 叶阿莉, 江萍, 陈宜张. 糖皮质激素及其他甾体激素对大鼠下丘脑薄片释放精氨酸加压素的影响[J]. 生理学报 1995; 47 (2): .

Liu Xiu, Wang Chunan, Ye Ali, Jiang Ping, Chen Yizhang. Effect of glucocorticoids and other steroids on arginine vasopressin release from rat hypothalamic slices. Acta Physiol Sin 1995; 47 (2): (in Chinese with English abstract).