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中枢注射U-50, 488H对肾水钠钾的排出及PVH内TH-IR神经元活性的影响

狄安稞, 黄龙

大连医科大学生理教研室. 辽宁, 大连 116023

摘要

乙醇麻醉的大鼠下丘脑室旁核(PVH)微量注射K型阿片受体激动剂U-50、488H(5#mu#g/#mu#l)后20min内大鼠尿量开始增加(P<0.01), 持续约100min, 41-60min尿量增加达峰值(P<0.01)。PVH预先(10min)注射K型阿片受体阻断剂NBT(5#mu#g/#mu#l)可以阻断U-50、488H所产生的利尿效应(P<0.0 1)。第三脑室注射U-50、488H(10#mu#g/10#mu#l)20min后, PVH酪氨酸羟化酶免疫反应阳性神经元数量减少,染色强度减弱, 于注药后50min变化最为显著,100min时已恢复正常。图3表1参25

关键词： U－50,488H; NBT; PVH; 尿量; 尿钠; 尿钾; TH－IR神经元

Effect of U-50, 488H administered centrally in rats on urination and activity of TH-IR neurons in PVH

Di Anke, Huang Long

Department of Physiology, Dalian Medical University. Dalian 116023, Liaoning

Abstract

The effect of a kappa--receptor agonist U--50, 488H on urine flow and the activity of TH--R (tyrosine hydroxylase--immunoreactivity) neurons in PVH (Paraventricular nucleus of the hypothalamus) was studied in ethanol--anesthetized rats. U--50, 488H (4#mu#g/1) #mu#l) injected into PVH induced a potent diuretic response with no significant aetion on mean arterial pressure and heart rate. The diuretic response began within 20 min after injection, reached a peak value 41--60 min later and subsided at about 100 min. this diuretic response could be blocked by pretreatment with kappa--receptor antagonist NBT (Nor--Binaltorphimine Tetrapydrate). Both the number of neurons and staining density of TH--IR in the PVH tended to decrease at 20 min after the injection of U--50, 488H (10#mu#g/10#mul) into the third cerebroventricle. The effect was most evident at 50 min and recovered at about 100 min after injection.

Key words: U-50,488H;NBT;PVH;TH-IR neurons;Urine flow;urinary Na~(+);Urinary K~(+)

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引用本文：

狄安稞, 黄龙. 中枢注射U-50, 488H对肾水钠钾的排出及PVH内TH-IR神经元活性的影响[J]. 生理学报 1995; 47 (2): .

Di Anke, Huang Long. Effect of U-50, 488H administered centrally in rats on urination and activity of TH-IR neurons in PVH. Acta Physiol Sin 1995; 47 (2): (in Chinese with English abstract).