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水稻胚乳甲壳素结合蛋白的cDNA克隆

杨日耀, 王彤宇, 唐锡华

中科院上海植物生理研究所. 上海(200032)

摘要

经3次筛选后仍保持阳性免疫反应的两个cDNA克隆表达产物cRRY1和cRRY2, 能与纯化的水稻 胚乳甲壳素结合蛋白起反应。用EcoRI和NotI切割cRRY1和cRRY2的DNA, 产物中分别含有长为1.6和1.0kb的cDNA插入片段。图3参8(S5)

关键词： 水稻胚乳甲壳素结合蛋白; cDNA克隆; 插入片段; 抗体筛选

The cDNA clones of chitin-binding-protein from rice seed endosperm

Yang Riyao, Wang Tongyu, Tang Xihua

Shanghai Institute of Plant Physiology, The Chinese Academy of Sciences. Shanghai

Abstract

Two cDNA clones of chitin-binding-protein from rice seed endosperm(ES-CBP): CRRY1 and CRRY2 were screened and isolated with the ES-CBP specific antibody from seed cDNA library(constructed in #lambda#gt-22A). The antiserum(selected after gene expression)can react with the purified ES-CBP. Furthermore, DNA of the positive positive clones were extracted and digested by EcoR I -Not I. Agarose-electrophoresis indicates that the size of the inserted fragments are 1.6kb and 1.0kb respectively.

Key words: ES-CBP;cDNA clone;Insert fragment;Antiserum screening

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引用本文：

杨日耀, 王彤宇, 唐锡华. 水稻胚乳甲壳素结合蛋白的cDNA克隆[J]. 生理学报 1994; 46 (4): .

Yang Riyao, Wang Tongyu, Tang Xihua. The cDNA clones of chitin-binding-protein from rice seed endosperm. Acta Physiol Sin 1994; 46 (4): (in Chinese with English abstract).