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蚕豆(Vicia faba L.)叶肉细胞中ABA的胶体金免疫电镜定位

贾文锁, 王学臣, 娄成后

北京农业大学生物学院. 北京(100094)

摘要

利用胶体金免疫电镜定位技术对蚕豆叶肉细胞中ABA定位的研究表明, 在以ABA抗体处理的切片中, 叶绿体有大量的金颗粒标记, 细胞质和细胞核也有金颗粒标记, 但液泡和细胞壁中没有金颗粒标记。免疫染色前用胰蛋白酶处理可显著增强金颗粒标记密度, 而不用EDC固定或 以免疫前兔血清处理的切片中几乎没有金颗粒标记。该实验为蚕豆叶肉细胞中ABA的分布提 供了直接的证据并说明了该技术是研究ABA定位的一种可靠的方法。图版2参8

关键词： ABA; 胶体金免疫电镜; 免疫定位

Immunogold electron-microscopy localization of abscisic acid in mesophyll cells of Vicia faba L.

Jia Wensuo, Wang Xuechen, Lou Chenghou

College of Biological Science, Beijing Agricultural University. Beijing

Abstract

With immunogold electron microscopy the study on the localization of abscisic acid (ABA) in mesophyll cell of Vicia faba has been undertaken. The results showed that, in thin sections treated with ABA antibody, chloroplasts, cytosolic compartments and the nuclei were labelled by gold particles, but chloroplasts were labelled by more gold particles. However, no gold particles were found in cell wall and vacuoles. Treating the sections with trypsin before immunostaining could increase the gold particle density substantially. The sections with no EDC fixation or treated with preimmune rabbit serum were devoid of gold particle label.

Key words: Abscisic acid;Immunogold electron microscopy;Immunelocalization

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引用本文：

贾文锁, 王学臣, 娄成后. 蚕豆(Vicia faba L.)叶肉细胞中ABA的胶体金免疫电镜定位[J]. 生理学报 1994; 46 (4): .

Jia Wensuo, Wang Xuechen, Lou Chenghou. Immunogold electron-microscopy localization of abscisic acid in mesophyll cells of Vicia faba L.. Acta Physiol Sin 1994; 46 (4): (in Chinese with English abstract).