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从睡眠剥夺树qu(TBC)尿液提取内源性“睡眠因子”(sleep factor)的研究

刘世熠, 张文远, 李海燕, 戴秀菊, 章懿, 徐杰诚, 陈玲玲, 王思清, 钱翠鹤

中国科学院上海生理研究所；中国科学院上海有机化学研究所

摘要

从睡眠剥夺(SD)48—72h的灵长类原宗(primitive stock) Tupaia belangeri chinensis (TBC)提取内源性“睡眠因子” (sleep factor)S2C和S4B。收集的尿液经超滤, 清液冻干经Sephadex G15分离得到Fraction Ⅰ—Ⅴ。活性测定发现Fraction-Ⅲ(S2C)呈现显著δ-增强促眠效应。经Sephadex G25和Sephadex LH20进一步净化的S4B也呈现显著δ-增强促眠效应。图1表2参14

关键词： 睡眠因子; 氨基酸; 睡眠剥夺; 树鼩

Studies on the isolation of endogenous sleep factors from Tupaia belangeric chinensis (TBC) after sleep deprivation

Liu Shiyi, Zhang Wenyuan, Li Haiyan, Dai Xiuju, Zhang Yi, Xu Jiecheng, Chen Lingling, Wang Siqing, Qian Cuihe

Shanghai Inst. Physiol., Acad. Sin.；Shanghai Inst. Org. Chem., Acad. Sin.

Abstract

Key words: Sleep factors;Amino-acids;Sleep deprivation(SD);Tupaia belangeri chinensis(TBC)

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引用本文：

刘世熠, 张文远, 李海燕, 戴秀菊, 章懿, 徐杰诚, 陈玲玲, 王思清, 钱翠鹤. 从睡眠剥夺树qu(TBC)尿液提取内源性“睡眠因子”(sleep factor)的研究[J]. 生理学报 1994; 46 (1): .

Liu Shiyi, Zhang Wenyuan, Li Haiyan, Dai Xiuju, Zhang Yi, Xu Jiecheng, Chen Lingling, Wang Siqing, Qian Cuihe. Studies on the isolation of endogenous sleep factors from Tupaia belangeric chinensis (TBC) after sleep deprivation. Acta Physiol Sin 1994; 46 (1): (in Chinese with English abstract).