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细胞内外钙浓度变化不影响酪氨酸抗hCG致孕酮生成作用

田淑君, 王启发, 程治平

哈尔滨医科大学生殖内分泌研究室. 黑龙江,哈尔滨(150086)

摘要

取经PMSG-hCG处理的未成年雌性大鼠卵巢, 用胶原酶-DNA酶消化, 制得黄体细胞悬浮液, 预孵育1h后加入各种处理因素, 继续孵育2h, 用放射免疫方法测孵育液中孕酮的量。孵育液中含有高钙或高钾或加入A23187时均可增加黄体细胞基础及hCG诱导的孕酮生成量。相反,减少钙的浓度或 加入EGAT或戊脉胺, 孕酮生成量则明显减少。酪氨酸抑制hCG刺激的孕酮生成,但对高钙、高钾和A23187增加孕酮的作用无影响, 并对上述三者分别与hCG同时作用所致孕 酮生成增加也无影响。提示: 大鼠黄体细胞孕酮生成依赖于细胞内外的钙; 细胞内外钙浓度的变化不影响酪氨酸抗hCG致孕酮生成作用; 钙与hCG使孕酮增加的作用可能是通过不同机制。图8参7

关键词： 钙; 酪氨酸; 孕酮; 黄体细胞

Variation in extracellular/intracellular Ca~(2+) does not affect tyrosine (Tyr) suppressive effect on hCG-induced progesterone production by rat corpus luteal cells in vitro

Tian Shujun, Wang Qifa, Cheng Zhiping

The Reproductive Endocrinology Laboratory, Harbin Medical University. Harbin, Heilongjiang

Abstract

Ovaries of immature female rats primed with PMSG-hCG were digested with collagenase-DNAase solution to obtain the corpus luteal cell suspensions. After pre-incubation for 1h, luteal cell suspensions were then incubated with different factors for 2h, The progesterone contents in the incubates were measured by RIA. It was demonostrated that high Ca~(2+)/high K~(+)/A23187 significantly enhanced both the basal or hCG-induced progesterone production by rat luteal cells. To the contrast, decreased Ca~(2+) concentration in medium/EGTA/verapamil had inhibitory effect on progesterone production in the presence of hCG.Tyr had suppressive effect on hCG-induced progesterone production. but not in the presence of high Ca~(2+)/high K~(+)/A23187. It suggested that progesterone production by luteal cells of rat is influenced by concentration. Yet, variation in extra/intra-cellular Ca~(2+) does not affect Tyr suppressive effect on hCG-induced progesterone production.

Key words: Calcium;Tyrosine;Progesterone;Corpus luteum cells

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引用本文：

田淑君, 王启发, 程治平. 细胞内外钙浓度变化不影响酪氨酸抗hCG致孕酮生成作用[J]. 生理学报 1994; 46 (4): .

Tian Shujun, Wang Qifa, Cheng Zhiping. Variation in extracellular/intracellular Ca~(2+) does not affect tyrosine (Tyr) suppressive effect on hCG-induced progesterone production by rat corpus luteal cells in vitro. Acta Physiol Sin 1994; 46 (4): (in Chinese with English abstract).