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一氧化氮合成酶(NOS)基因表达的半定量检测及其运用

张晨晖, 李倩虹, 张继峰, 周洪, 张新波, 汤健

北京医科大学心血管基础研究所. 北京(100083)

摘要

参照Martin(1993)定量RT-PCR方法, 建立了一种灵敏、简捷、特异的定量NOS mRNA测定方法; 证明了NOS mRNA不仅存在于脑组织, 亦广泛分布于心、肾、肺和肝组织中, 其中以脑组织含量最高, 肾、心次之。除内皮细胞外, 平滑肌细胞中亦有NOS基因的表达;还观察到自发性高血压大鼠的脑、肾、肝和平滑肌细胞中NOS mRNA水平下降, 提示NOS基因表达受抑,可能与高血压的痛因密切相关。图6参14

关键词： 一氧化氮合成酶; 基因表达; 自发性高血压大鼠; 半定量PCR

Semiquantitative detection and application about the expression of nitric oxide synthase gene

Zhang Chenhui, Li Qianhong, Zhang Jifeng, Zhou Hong, Zhang Xinbo, Tang Jian

Cardiovascular Basic Institute, Beijing Medical University. Beijing；China(100083)

Abstract

Using the Reverse Transcriotion (RT)-Polymerase Chain Reaction(PCR) method of Martin (1993) for semiquantitation determinating of NOS gene, it was found that NOS mRNA is not only existed in brain, but also distributed extensively in heart, kidney, lung and liver. Among of them, NOS mRNA levels were highest in the brain, followed in descending order by kidney, heart. In addition to endothelial cell, NOS gene was also highly expressed in smooth muscle cells, suggesting that they may be an important site of NOS in organism. Furthermore, NOS mRNA levels were found to decrease significately in brain, kidney, liver and smooth muscle cell in spontaneous hypertensive rat.

Key words: Nitric oxide synthase;Gene expression;Spontaneous hypertensive rat; Semiquantitative polymerase chain reaction

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引用本文：

张晨晖, 李倩虹, 张继峰, 周洪, 张新波, 汤健. 一氧化氮合成酶(NOS)基因表达的半定量检测及其运用[J]. 生理学报 1994; 46 (4): .

Zhang Chenhui, Li Qianhong, Zhang Jifeng, Zhou Hong, Zhang Xinbo, Tang Jian. Semiquantitative detection and application about the expression of nitric oxide synthase gene. Acta Physiol Sin 1994; 46 (4): (in Chinese with English abstract).