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导水管周围灰质微量注射阿片肽拮抗剂阻断甲醛致痛时脊髓背角P物质的变化

杜俊辉, 何莲芳

上海医科大学医学神经生物学国家重点实验室. 上海(200032)

摘要

导水管周围灰质内微量注射纳洛酮和β-内啡肽抗血清可以易化动物脚掌注射福尔马林引起的痛反应, 并阻断脊髓背角P物质的增加。提示伤害性刺激可以引起中脑导水管周围灰质内 β-内啡肽的释放, 与相应的阿片受体作用, 调制脊髓背角P物质的释放。图2表1参8

关键词： 纳洛酮; β－内啡肽; 中脑导水管周围灰质; P物质; 脊髓背角; 免疫组织化学; 图象分析; 福尔马林致痛

Microinjection of opioid antagonist into periaqueductal gray blocks formalin-induced alterations of substance P in spinal dorsal horn

Du Junhui, He Lianfang

Department of Neurobiology, Shanghai Medical University. Shanghai

Abstract

Using formalin injected into the rat^s hind paw as nociceptive stimulation, the effect of naloxone and #beta#-endorphin(#beta#-EP)antiserum microinjected into the periaqueductal gray (PAG) on the level of substance P like immunoreactivity (SPLI) of the spinal dorsal horn was observed. After formalin injection, a significant increase in SPLI was found as compared with the control(P<0.01). After naloxone or #beta#-EP antiserum microinjection, SPLI level in the dorsal horn was decreased (P<0.05). It is thus suggested that nociceptive stimulation induce the release of #beta#-EP in the PAG, which, in turn, modulate SP release in the spinal dorsal horn.

Key words: Naloxone;#beta#-endorphin;Periaqeductal gray;Substance P;Spinal dorsal horn;Immunohistochemistry;Image processing;Formalin test

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引用本文：

杜俊辉, 何莲芳. 导水管周围灰质微量注射阿片肽拮抗剂阻断甲醛致痛时脊髓背角P物质的变化[J]. 生理学报 1994; 46 (4): .

Du Junhui, He Lianfang. Microinjection of opioid antagonist into periaqueductal gray blocks formalin-induced alterations of substance P in spinal dorsal horn. Acta Physiol Sin 1994; 46 (4): (in Chinese with English abstract).