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枸杞果实铁型超氧物歧化酶的纯化及性质

程光宇, 魏锦城, 吴国荣, 刘子列, 邹玉珍

南京师范大学生物系. 江苏, 南京(210024)

摘要

枸杞果实Fe-SOD粗提液经硫酸铵盐析、离子交换柱层析及凝胶过滤,纯化到电泳单斑点均一程度。纯化的Fe-SOD分子量为44.6kD,亚基分子量为22.0kD。金属元素分析表明, 每分子酶含1个Fe原子。该酶在紫外区最大吸收值为278nm。H_(2)O_(2)明显抑制该酶活性, KCN对酶活性无影响。该酶氨基酸组成与高等植物和蓝绿藻的Fe-SOD相似,但具较高甘氨酸; 酸性氨基酸与碱性氨基酸比值高于高等植物,与低等植物及原核生物相近。图6表3参22

关键词： 枸杞; 果实; 铁型超氧物歧化酶; 纯化; 性质

Isolation and characterization of an iron-containing superoxide dismutasefrom Lycium chinense fruit

Cheng Guangyu, Wei Jincheng, Wu Guorong, Liu Zilie, Zou Yuzhen

Department of Biology, Nanjing Normal University, Nanjing,Jiangsu

Abstract

Iron-containing superoxide dismutase was found in the crude extract fromfruit of Lycium chinense Mill, the enzyme was isolated and purified tohomogeneity by ammonium sulfate fractionation, DEAE-Sephadex A-50chromatography and Sephadex G-100 gel filtration. The enzyme had a specificactivity of about 1000U/mg, and exhibited one absorption maximum in theultraviolet at 278nm. The purified enzyme was stable up to at least 65℃. Theenzyme activity could be inhibited by H_(2)O_(2) but was unaffected byKCN. The enzyme has a relative molecular mass of about 44.6kD and is composedof two equal subunits. The molecular mass of the subunits has been found to be22.0kD. The Fe-enzyme contains about 1 gram atom of Fe per dimer and containsmore glycine residues than the Fe-SOD from procaryotes and other eucaryotes.

Key words: Lycium chinense Mill;Iron-containing superoxide dismutase;Fruit;Purification;Property

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引用本文：

程光宇, 魏锦城, 吴国荣, 刘子列, 邹玉珍. 枸杞果实铁型超氧物歧化酶的纯化及性质[J]. 生理学报 1994; 46 (2): .

Cheng Guangyu, Wei Jincheng, Wu Guorong, Liu Zilie, Zou Yuzhen. Isolation and characterization of an iron-containing superoxide dismutasefrom Lycium chinense fruit. Acta Physiol Sin 1994; 46 (2): (in Chinese with English abstract).