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一种改进的小鼠局灶性脑缺血神经症状定量评价方法

魏尔清, 朱朝阳, 徐秋琴, 俞月萍, 朱也飞, 郑敏之

浙江大学医学院药理学教研室.浙江,杭州 310031

摘要

该文旨在建立一种客观评价小鼠局灶性脑缺血神经症状的定量方法。在大脑中动脉阻塞诱导局灶性脑缺血后24h,采用悬挂试验分别测定转动的平均角和优势角以及转动次数,并用爬板试验测定小鼠攀爬角度;分析定量测定指标与脑梗死体积、神经元密度的相关性,并与经典的行为学评价方法比较。还以此法观察抗脑缺血药pranlukast,4-氧-8[对-(4-苯丁氧基)苯甲酰氨基]-2-(5-四氮基)-4H-1-苯并吡喃半水化合物(ONO-1078)的作用。结果显示,脑缺血小鼠各项指标均有显著改变,定量评价总分与脑梗死体积、神经元密度减少密切相关,与经典行为学评分之间也密切相关。ONO-1078可显著抑制缺血损伤,并降低定量评价总分。因此,该方法可反映局灶性脑缺血神经症状变化,具有客观、定量、操作简便、无损伤的优点,并能用于评价抗脑缺血药物的作用。

关键词： 神经生物学; 局灶性脑缺血; 神经学检查; 偏瘫; 小鼠; {pranlukast 4-氧-8-[对-（4-苯丁氧基）苯甲酰氨基]-2-（5-四氮基）-4H-1-苯并吡喃半水化合物}; （ONO-1078）

An improved quantitative method for evaluating neurological deficits in mice with focal cerebral ischemia

Wei Erqing, Zhu Chaoyang, Xu Qiuqin, Yu Yueping, Zhu Yefei, Zheng Minzhi

Department of harmacology,School of Medicine,Zhejiang University.Hangzhou 310031,Zhejiang

Abstract

We found that the variables measured by both quantitative and typical behavioral methods significantly changed in the ischemic mice, and correlated with the infarct volumes and neuron densities. The quantitative variables well correlated with those of typical behavioral assessment, too. ONO-1078 inhibited ischemic injury and reduced the total scrores of quantitative assessment. Thus, the quantitative method we developed is useful in evaluating neurological deficits of focal cerebral ischemia with the advantages of objectivity, quantification, simplicity and non-invasion, and can be used in the evaluation of neuroprotective effects of drugs.

Key words: Nneurobiology;Focal cerebral ischemia;Neurological examination;Hemiparesis;Mouse;{pranlukast，4-oxo-8-[p-(4-phenylbutyloxy) benzoylamino〕-2-(tetrazol-5-yl)-4H-1-benzopyran hem ihydrate};(ONO-1078)

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引用本文：

魏尔清, 朱朝阳, 徐秋琴, 俞月萍, 朱也飞, 郑敏之. 一种改进的小鼠局灶性脑缺血神经症状定量评价方法[J]. 生理学报 2003; 55 (6): .

Wei Erqing, Zhu Chaoyang, Xu Qiuqin, Yu Yueping, Zhu Yefei, Zheng Minzhi. An improved quantitative method for evaluating neurological deficits in mice with focal cerebral ischemia. Acta Physiol Sin 2003; 55 (6): (in Chinese with English abstract).