ISSN 0371-0874, CN 31-1352/Q

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番茄感染TMV诱导的#beta#-1,3-葡聚糖酶的纯化和性质

史益敏, 颜季琼, 费雪南, 许煜泉

上海农学院植物科学系;华东师范大学生物系

摘要

番茄系系统感染TMV诱导叶胞外#beta#-1, 3-葡聚糖酶活性升高。番茄叶胞外提取液经冰 冻干燥浓缩、-20℃丙酮沉淀、CM-Sephadex C-25离子交换层析和PBE 94聚焦层析纯化, 获得PA-GE和SDS-PAGE均一的#beta#-1, 3-葡聚糖酶。在感染TMV的番茴茄叶中, #beta#-1, 3-葡聚糖酶活力大部分位于胞外, 它是番茄叶胞外提取液中主要的病原相关蛋白。图9表1参18

关键词: 番茄; 番茄花叶病毒; 葡聚糖酶

Purification and properties of a #beta#-1, 3-glucanase in tomato leaves infected with TMV (tobacco mosaic virus)

Shi Yimin, Yan Jiqiong, Fei Xuenan, Xu Yuquan

Dept. Plant Sci., Shanghai Agri. Coll.;Dept. Biol., E China Norm. Univ.

Abstract

Key words: Tomato;Tobacco mosaic virus;#beta#-1,3-glucanase

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引用本文:

史益敏, 颜季琼, 费雪南, 许煜泉. 番茄感染TMV诱导的#beta#-1,3-葡聚糖酶的纯化和性质[J]. 生理学报 1993; 45 (4): .

Shi Yimin, Yan Jiqiong, Fei Xuenan, Xu Yuquan. Purification and properties of a #beta#-1, 3-glucanase in tomato leaves infected with TMV (tobacco mosaic virus). Acta Physiol Sin 1993; 45 (4): (in Chinese with English abstract).