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人参总皂甙对人GM-CSF和GM-CSFR表达的调控

王莎莉, 陈笛, 王亚平, 刘永刚, 姜蓉

重庆医科大学基础医学院生理教研室.重庆 400016；重庆医科大学基础医学院组织胚胎学教研室.重庆 400016

摘要

研究人参总皂甙(TSPG)对人粒-巨噬细胞集落刺激因子(GM-CSF)和粒-巨噬细胞集落刺激因子受体#alpha#(GM-CSFR#alpha#)表达的影响。结果:(1)经TSPG(50#mu#g/ml)诱导制备的骨髓基质细胞、胸腺细胞、脾细胞、血管内皮细胞和单核细胞条件培养液可显著提高粒单系造血祖细胞(CFU-GM)的集落产率;(2)经TSPG(50#mu#g/ml)诱导后,上述细胞的GM-CSF蛋白(诱导24h)和mRNA(诱导12h)表达显著提高;(3)经TSPG(50#mu#g/ml)诱导24h骨髓造血细胞的GM-CSFR#alpha#蛋白表达增强;(4)经TSPG(50#mu#g/ml)刺激后2min,GM-CSFR#alpha#和Shc发生酪氨酸磷酸化,5min时达高峰,随后去磷酸化。上述结果表明,TSPG可能通过直接和/或间接途径促进淋巴细胞与骨髓基质细胞合成与分泌GM-CSF,诱导骨髓造血细胞表达GM-CSFR#alpha#,并刺激GM-CSFR#alpha#和Shc的酪氨酸可逆磷酸化,从而通过调控GM-CSF的信号转导过程,促进CFU-GM的增殖。

关键词： 人参总皂甙; 粒单系祖细胞; 粒-巨噬细胞集落刺激因子; 粒-巨噬细胞集落刺激因子受体α; 信号转导

Modulation of expression of human GM-CSF and GM-CSFR#alpha# by total saponins of Panax ginseng

Wang Shali, Chen Di, Wang Yaping, Liu Yonggang, Jiang Rong

Department of Physiology,College of Basic Medical Sciences,Chongqing Medical University.Chongqing 400016；China

Abstract

Bone marrow stomal cell (BMSC), thymocyte (TC), splenocyte (SC), endothelial cells (EC), and monocyte (MO) conditioned media prepared with TSPG (50 #mu#g/ml) could significantly enhance the proliferation ofo CFU-GM; the expressions of GM-CSF in protein and mRNA level in BMSC, TC, SC, EC and MO induced by TSPG (50 #mu#g/ml) were much higher than that of the control; the expression of GM-CSFR#alpha# protein in hematopoietic cells induced by TSPG (50 #mu#g/ml) was stronger than that of the control; TSPG (50 #mu#g/ml) could stimulate the transient tyrosine phosphorylation of GM-CSFR and Shc protein. We speculate that TSPG may directly and/or indirectly promote the stromal cells and lymphocytes to produce GM-CSF and other cytokine and induce bone marrow hematopoietic cells to express GM-CSF receptors (GM-CSFR#alpha#), leading to the regulation of the GM-CSFR-mediated signals transduction pathway and the proliferation of human CFO-GM.

Key words: Total saponins of Panax;Ginseng;CFU-GM;GM-CSF GM-CSFRα;Signal transduction

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引用本文：

王莎莉, 陈笛, 王亚平, 刘永刚, 姜蓉. 人参总皂甙对人GM-CSF和GM-CSFR表达的调控[J]. 生理学报 2003; 55 (4): .

Wang Shali, Chen Di, Wang Yaping, Liu Yonggang, Jiang Rong. Modulation of expression of human GM-CSF and GM-CSFR#alpha# by total saponins of Panax ginseng. Acta Physiol Sin 2003; 55 (4): (in Chinese with English abstract).