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高产人参寡糖素培养细胞克隆系的诱变筛选

罗建平, 郑光植, 甘烦远

中科院昆明植物研究所. 云南,昆明(650204)

摘要

紫外辐射能显著地降低人参培养细胞单细胞克隆的植板率。当紫外辐射悬浮细胞30s后, 细 胞克隆的植板率是对照组的21.43%。细胞克隆平板培养60d, 挑取克隆连续转移培养3次, 共获得克隆系122株, 经10代连续继代培养, 筛选到稳定高产寡糖素克隆系PGUA-08, 而且其 过氧化物酶同工酶谱特征也保持稳定。图5表2参11

关键词： 人参; 单细胞克隆; 克隆系; 寡糖素; 紫外诱变; 过氧化物酶同工酶

Selection of variants with high yield of oligosaccharin from Panaxginseng cultured cells

Luo Jianping, Zheng Guangzhi, Gan Fanyuan

Kunming Institute of Botany, The Chinese Academy of Sciences. Kunming,Yunnan

Abstract

Cultured cells of Panax ginseng were found to be highly sensitive to UV-irradiation, which could markedly decrease the plating efficiency of single cell cloning of P. ginseng cultured cells. After cultured cells were treated with UV-irradiation for 30 s, the plating efficiency was 21.4% of the control. Cell growth rate and oligosaccharin content of all 122 clone lines obtained from 165 clone lines through 3 times transplantation culture, chosen after 60 d of plating culture, were measured. Basing on the stability of clone lines obtained through 10 generations successive subculture, a stable clone line PGUA-08 with high yield of oligosaccharin had been selected. Moreover, the isozyme patterns of peroxidases of clone line PGUA-08 maintained almost unchanged during successive subculture. The growth rate of clone line PGUA-08 was 0.537 g DW~(-1)L~(-1)d~(-1), and was 46% higher than that of its parent, and its oligosaccharin content and yield were 17.16% DW and 2.764 g/L, respectively, 1.81 and 2.62 times that of its parent, respectively.

Key words: Panax ginseng single cell clone;Clone lines;Oligosaccharins;UV--irradiation;Peroxidase isozyme;

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引用本文：

罗建平, 郑光植, 甘烦远. 高产人参寡糖素培养细胞克隆系的诱变筛选[J]. 生理学报 1994; 46 (4): .

Luo Jianping, Zheng Guangzhi, Gan Fanyuan. Selection of variants with high yield of oligosaccharin from Panaxginseng cultured cells. Acta Physiol Sin 1994; 46 (4): (in Chinese with English abstract).