Issue Archive

CircRNA-0028171 regulates arsenic trioxide-induced apoptosis in vascular endothelial cells

WU Ji-Chen¹, JIN Sai-Di², SONG Jia-Hang², LIU Xin-Qi², MA Wen-Jun¹, CHANG Lin¹, GUAN Xiao-Xiang², ZHANG Ming-Yu², LIU Jia-Qi¹, FU Hui¹, WANG Ying³, XU Chao-Qian^2,*

¹College of Pharmacy, Mudanjiang Medical University, Mudanjiang 157400, China；²College of Pharmacy, Harbin Medical University, Harbin 150081, China；³College of Basic Medicine, Mudanjiang Medical University, Mudanjiang 157400, China

Abstract

The present study was aimed to investigate the effects of circRNA-0028171 on the apoptosis of vascular endothelial cells induced by arsenic trioxide (As2O3). Human umbilical vein endothelial cells (HUVECs) were treated with 0–15 μmol/L As2O3 for 24 h. Then, cellular viability was measured by MTT assay. The expression levels of circRNA-0028171, Bcl-2 and Bax mRNA were detected by real-time quantitative PCR. Bcl-2/Bax protein ratio was detected by Western blot. Whether circRNA-0028171 was involved in the regulation of HUVECs by As2O3 was investigated by transfection with overexpression plasmid of circRNA-0028171 and siRNA. The results showed that compared with the control group, As2O3 group showed decreased cellular viability, reduced Bcl-2/Bax mRNA and protein ratios, and significantly lower expression of circRNA-0028171. Overexpression of circRNA-0028171 inhibited apoptosis of HUVECs induced by As2O3. Knockdown of circRNA-0028171 by siRNA promoted As2O3-induced apoptosis in HUVECs. These results suggest that circRNA-0028171 is involved in the vascular endothelial cell apoptosis induced by As2O3.

Key words: circular RNAs; vascular endothelial cells; arsenic trioxide; apoptosis

Received: 　　Accepted:

Corresponding author: 许超千　　E-mail: xuchaoqian@ems.hrbmu.edu.cn

DOI: 10.13294/j.aps.2022.0047

Citing This Article：

WU Ji-Chen, JIN Sai-Di, SONG Jia-Hang, LIU Xin-Qi, MA Wen-Jun, CHANG Lin, GUAN Xiao-Xiang, ZHANG Ming-Yu, LIU Jia-Qi, FU Hui, WANG Ying, XU Chao-Qian. CircRNA-0028171 regulates arsenic trioxide-induced apoptosis in vascular endothelial cells. Acta Physiol Sin 2022; 74 (5): 763-772 (in Chinese with English abstract).