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The distribution of Mas-related G protein-coupled receptor A in cerebrospinal fluid-contacting nucleus of normal rats and its up-regulation in neuropathic pain

CHEN Yu-Feng^1,2, TIAN En-Qi^1,2, WANG Guo-Ping¹, ZHOU Fang², ZHANG Li-Cai^2,*

¹Department of Anesthesiology, Changzhi People’s Hospital, Changzhi 046000, China；²Jiangsu Provincial Key Laboratory of Anesthesiology, Xuzhou Medical University, Xuzhou 221000, China

Abstract

This study was aimed to observe the distribution of Mas-related G protein-coupled receptor A (MrgA) in cerebrospinal fluid (CSF)-contacting nucleus of normal rats and its expression in neuropathic pain, and to provide morphological evidence for CSF- contacting nucleus to participate in neuropathic pain. The model of neuropathic pain with chronic constriction injury (CCI) of the sciatic nerve was made in Sprague-Dawley rats. The thermal withdrawal latency (TWL) and mechanical withdrawal threshold (MWT) were measured. The expressions of MrgA in the CSF-contacting nucleus were examined by double labeling with immunofluorescent staining. The results showed that on the 5th, 7th, 10th and 14th days, the values of MWT and TWL in CCI group were all lower than those in sham group (P < 0.05). MrgA was found to be distributed in CSF-contacting nucleus of normal rats; and the expression was markedly up-regulated in rats at the peak of neuropathic pain. Our data suggest that CSF-contacting nucleus may participate in neuropathic pain through the MrgA-mediated signaling pathway.

Key words: CSF-contacting nucleus; MrgA; neuropathic pain

Received: 　　Accepted:

Corresponding author: 张励才　　E-mail: licaizhang001@163.com

DOI: 10.13294/j.aps.2022.0044

Citing This Article：

CHEN Yu-Feng, TIAN En-Qi, WANG Guo-Ping, ZHOU Fang, ZHANG Li-Cai. The distribution of Mas-related G protein-coupled receptor A in cerebrospinal fluid-contacting nucleus of normal rats and its up-regulation in neuropathic pain. Acta Physiol Sin 2022; 74 (3): 353-358 (in Chinese with English abstract).