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Behavioral-electrophysiological observation of the involvement of dopamine D1 receptor of the rat anterior cingulate cortex in the regulation of pain-related emotion 

DU Xiang-Xin¹, ZHANG Li-Na¹, ZHANG Yu-Tong¹, HAO Na¹, GUO Xia¹, ZHAO Xin¹, WANG Zhi-Hua², ZHANG Yu^1,*

¹Department of Physiology, Key Laboratory of Cell Physiology, Ministry of Education, Shanxi Medical University, Taiyuan 030001, China；²Department of Pathology, Shanxi Medical University, Taiyuan 030001, China

Abstract

The present study was aimed to explore the involvement of dopamine D1 receptor of the anterior cingulate cortex (ACC) in the regulation of chronic inflammatory pain-related emotion. On the first day, the rats were acclimated to the environment and the baseline indices were measured. On the second day, the rats were administered with the dopamine D1 receptor antagonist SCH-23390 or agonist SKF38393 in the ACC, and then they were subcutaneously injected with complete Freund’s adjuvant (CFA, 0.08 mL) in the left hind paw to establish conditioned place avoidance (CPA) response after pairing with specific environment. On the third day, the CPA response and the firing frequency of ACC neurons were observed synchronously, and the open-field behavior, mechanical pain behavior and paw withdrawal latency (PWL) tests were also observed subsequently. In other experiments, rats were given subcutaneous injection of normal saline (NS) on the left hind paw after SCH-23390 or SKF-38393 was administered in the ACC, and then the same observations were performed. The results showed that: (1) Compared with the control group, the PWL and mechanical pain thresholds of rats injected with CFA on the left hind paw were significantly decreased (P < 0.05); (2) The residence time of rats injected with CFA in the “pain environment” and open field center was significantly shortened (P < 0.05); (3) Pre-injection of antagonist SCH-23390 in ACC (10 μg) alleviated the anxiety-like negative behavior response induced by CFA (P < 0.05) and reversed CFA-induced increases of discharge frequency of ACC neurons (P < 0.05); (4) Pre-injection of agonist SKF-38393 in the ACC (10 μg) induced CPA-like behavioral response in rats injected with NS in the left hind paw, and increased the firing frequency of ACC neurons (P < 0.05); (5) Immunofluorescence detection showed that dopamine D1 receptor and NMDA receptor were co-expressed in the same neuron. These results suggest that inhibition of dopamine D1 receptor in ACC can alleviate the negative emotional response induced by persistent pain.  

Key words: dopamine D1 receptor; anterior cingulate cortex; complete Freund’s adjuvant; pain-related emotions; multi-channel electrophysiology

Received: 　　Accepted:

Corresponding author: 张宇　　E-mail: zhyucnm@163.com

DOI: 10.13294/j.aps.2022.0026

Citing This Article：

DU Xiang-Xin, ZHANG Li-Na, ZHANG Yu-Tong, HAO Na, GUO Xia, ZHAO Xin, WANG Zhi-Hua. Behavioral-electrophysiological observation of the involvement of dopamine D1 receptor of the rat anterior cingulate cortex in the regulation of pain-related emotion . Acta Physiol Sin 2022; 74 (2): 155-164 (in Chinese with English abstract).