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Effect of shear stress on eicosanoid metabolism in endothelial cells by metabolomic approach

ZHAO Yin-Jiao^1,2, LIU Le^1,2, YAO Liu^1,2, ZHU Yi^1,2, ZHANG Xu^1,2,*

¹Department of Physiology and Pathophysiology, Tianjin Medical University, Tianjin 300070, China；²Key Laboratory of Metabolic Cardiovascular Disease, Tianjin Medical University, Tianjin 300070, China

Abstract

The article aims to study the effect and mechanism of shear stress on eicosanoids produced by the metabolism of polyunsaturated fatty acids in endothelial cells. First, human umbilical vein endothelial cells were treated by control (Static), laminar shear stress (LSS) and oscillatory shear stress (OSS) for 6 h. Then the endothelial cells were incubated with fresh M199 medium for 3 h, and the cell culture medium was collected. Ultra-performance liquid chromatography-mass spectrometer was used to detect the level of eicosanoid metabolites secreted by endothelial cells. The results showed that under different shear stress, the level of eicosanoid metabolites were changed significantly. We found 10 metabolites were significantly up-regulated by OSS compared with those in LSS group, including PGD2, PGE2, PGF2α and PGJ2 produced by cyclooxygenase; 11-HETE, 15-HETE, 13-HDoHE produced by lipoxygenase or spontaneous oxidation; 12,13-EpOME, 9,10-EpOME, 9,10-DiHOME produced by cytochrome P450 oxidase and soluble epoxide hydrolase. The transcription levels of these up-regulated eicosanoids metabolic enzyme-related genes were also increased in vitro and in vivo. These results indicate that OSS may promote the increase of metabolites by up-regulating the transcription level of metabolic enzyme-related genes, which playing a key role in the development of atherosclerosis. This study reveals the effect of shear stress on eicosanoid metabolism in endothelial cells, which provides a novel supplement to the systems biology approach to study systemic hemodynamics.

Key words: shear stress; eicosanoids; endothelial cells; metabolomics; atherosclerosis

Received: 2021-03-29　　Accepted: 2021-05-14

Corresponding author: 张栩　　E-mail: xuzhang@tmu.edu.cn

DOI: 10.13294/j.aps.2021.0035

Citing This Article：

ZHAO Yin-Jiao, LIU Le, YAO Liu, ZHU Yi, ZHANG Xu. Effect of shear stress on eicosanoid metabolism in endothelial cells by metabolomic approach. Acta Physiol Sin 2021; 73 (4): 539-550 (in Chinese with English abstract).