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Splenic tyrosine kinase promotes pulmonary angiogenesis in rats with hepatopulmonary syndrome 

GAO Wei-Zhong¹, YANG Yi-Hui¹, DAN Ling², ZHU Xi-Wen^2,*

¹Department of Anesthesiology, the First People’s Hospital of Zunyi, Zunyi 563000, China；²Department of Anesthesiology, Second Affiliated Hospital of Chongqing Medical University, Chongqing 400010, China

Abstract

The present paper was aimed to study the role of spleen tyrosine kinase (Syk) in angiogenesis in hepatopulmonary syndrome (HPS) and the underlying mechanism. Sprague Dawley (SD) rats were randomly divided into three groups: sham operation group (sham group), common bile duct ligation (CBDL) 5-week group (5W group) and R788 intervention group (R788 group). HPS model was established by CBDL. Rats in R788 group were intraperitoneally injected with R788 (20 mg/kg) once daily to week 5 after CBDL operation. The protein expression levels and distribution of Syk, p-Erk1/2, and p-Akt in lung tissue were detected by Western blot and immunohistochemistry. Immunofluorescence staining was used to observe the location of Syk expression and the number of angiogenesis in lung tissue. The results showed that, compared with sham group, 5W group exhibited up-regulated protein expression level of Syk, increased phosphorylation levels of Erk1/2 and Akt, and increased number of pulmonary microvessels. Compared with 5W group, R788 group exhibited down-regulated protein expression level of Syk, decreased phosphorylation levels of Erk1/2 and Akt, and decreased number of pulmonary microvessels. These results suggest that Syk may promote pulmonary angiogenesis in HPS model rats by activating downstream Erk1/2 and Akt signaling pathways, which provides a theoretical basis and potential drug therapeutic targets for the clinical treatment of HPS.

Key words: spleen tyrosine kinase; hepatopulmonary syndrome; angiogenesis; ERK1/2; Akt

Received: 2019-11-07　　Accepted: 2020-09-21

Corresponding author: 朱稀雯　　E-mail: 125625844@qq.com

DOI: 10.13294/j.aps.2020.0087

Citing This Article：

GAO Wei-Zhong, YANG Yi-Hui, DAN Ling, ZHU Xi-Wen. Splenic tyrosine kinase promotes pulmonary angiogenesis in rats with hepatopulmonary syndrome . Acta Physiol Sin 2020; 72 (6): 785-792 (in Chinese with English abstract).