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CCL2 promotes angiogenesis of primary rat cardiac microvascular endothelial cells

LOU Qiang^1,*, LIU Yong-Lian¹, ZHANG Shu-Man², LI Yuan-Yuan¹, HUANG Xiao-Feng¹

¹National and Local Joint Laboratory for Antibody Drug Development Techniques, Basic Medical School of Henan University, Kaifeng 475004, China；²The Huaihe Clinical College of Henan University, Kaifeng 475000, China

Abstract

The aim of the present study was to investigate the role of chemokine CCL2 in angiogenesis of primary adult rat cardiac microvascular endothelial cells (CMEC). The rat CMECs were isolated and identified through morphology examination and immunostaining with CD31 and factor VIII antibodies. The angiogenesis of CMEC on Matrigel was evaluated at different time points. The expression and secretion of CCL2 during the process of angiogenesis was detected by real-time RT-PCR and ELISA, respectively. The results showed that, the primary rat CMEC was isolated successfully, and the angiogenesis of CMEC was significantly induced after Matrigel treatment for 4 h. The expression of CCL2 and CCR2 were increased during angiogenesis, and the secretion of CCL2 was detected after 2 h of angiogenesis and reached the peak concentration of 1 588.1 pg/mL after 4 h. Either CCL2 blocking antibody or CCR2 antagonist significantly reduced the angiogenesis of CMEC. These results suggest that CCL2 is secreted during the process of angiogenesis of CMEC, and CCL2/CCR2 signaling pathway may play an important role in promoting angiogenesis.

Key words: CCL2; primary rat cardiac microvascular endothelial cells; angiogenesis

Received: 2019-08-07　　Accepted: 2020-03-05

Corresponding author: 娄强　　E-mail: qiang_lou@126.com

DOI: 10.13294/j.aps.2020.0050

Citing This Article：

LOU Qiang, LIU Yong-Lian, ZHANG Shu-Man, LI Yuan-Yuan, HUANG Xiao-Feng. CCL2 promotes angiogenesis of primary rat cardiac microvascular endothelial cells. Acta Physiol Sin 2020; 72 (4): 441-448 (in Chinese with English abstract).