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Establishment and application of a cell model for LRRC8A physiological characteristic study 

ZHOU Yan-Hong¹, ZHENG Kai², XIA Zhong-Xue¹, JIANG Xiao-Ming³, DI Wen-Hui¹, XU Lian-Xiu¹, YING Chao¹, HAO Feng^1,*

¹Laboratory Medical College, Jilin Medical College, Jilin 132013, China；²Management College, Jilin Medical College, Jilin 132013, China；³Pharmacy College, Beihua University, Jilin 132013, China

Abstract

The aim of the present study was to establish a cell model of  volume-regulated anion channel subunit LRRC8A and investigate the physiological characteristics of LRRC8A. The eukaryotic expression vectors of LRRC8A and YFP-H148Q/I152L were constructed and transfected into Fischer rat thyroid (FRT) cells by Lipofectamine 2000. The FRT cell lines co-expressing LRRC8A and YFP-H148Q/I152L were obtained by antibiotic screening. The expression of LRRC8A and YFP-H148Q/I152L in FRT cells was detected by the inverted fluorescence microscope. The fluorescence quenching kinetic experiment was done to verify the function and effectiveness of the cell model. Then the cell model was utilized to study the physiological characteristics of LRRC8A, such as the characteristics of anion transport, the opening of LRRC8A by osmotic pressure, the effect of anion transport velocity, and the effect of chloride channel inhibitors on LRRC8A anion channel. The results of the inverted fluorescence microscope showed that LRRC8A was expressed on the cell membrane and YFP-H148Q/I152L was expressed in the cytoplasm. The results of fluorescence quenching kinetic test showed that under the condition of low osmotic state, LRRC8A could transport some kinds of anions, such as iodine and chloride ions. Osmotic pressure played a key role in the regulation of LRRC8A volume-regulated anion channel opening. Chloride channel inhibitors inhibited ion transport of LRRC8A channel in a dose-dependent manner. It is suggested that LRRC8A has the characteristics of classic volume-regulated anion channels by using the cell model of FRT cells co-expressing LRRC8A and YFP-H148Q/I152L.

Key words: LRRC8A; volume-regulated anion channels; osmotic pressure; FRT cells

Received: 2018-08-29　　Accepted: 2019-03-11

Corresponding author: 郝峰　　E-mail: haof863@126.com

DOI: 10.13294/j.aps.2019.0045

Citing This Article：

ZHOU Yan-Hong, ZHENG Kai, XIA Zhong-Xue, JIANG Xiao-Ming, DI Wen-Hui, XU Lian-Xiu, YING Chao, HAO Feng. Establishment and application of a cell model for LRRC8A physiological characteristic study . Acta Physiol Sin 2019; 71 (4): 555-561 (in Chinese with English abstract).