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S100 calcium binding protein A16 promotes fat synthesis through endoplasmic reticulum stress in HepG2 cells 

KAN Jing-Bao¹, SHEN Ge-Qian¹, YANG Jie¹, TONG Pei¹, ZHANG Ri-Hua¹, LIANG Xiu-Bin², SU Dong-Ming², LI Dong³, LIU Yun^1,*

¹Department of Geratology, The First  Affiliated  Hospital  of  Nanjing  Medical  University, Nanjing  210029, China；²The Center of Metabolic Disease Research, Nanjing Medical University, Nanjing 210029, China；³Medicine, Affiliated Hospital of Nanjing University of Chinese Medicine, Nanjing 210029, China

Abstract

The aim of this study was to investigate the role of S100 calcium binding protein A16 (S100A16) in lipid metabolism in hepatocytes and its possible biological mechanism. HepG2 cells (human hepatoma cell line) were cultured with fatty acid to establish fatty acid culture model. The control model was cultured without fatty acid. Each model was divided into three groups and transfected with S100a16 over-expression, shRNA and vector plasmids, respectively. The concentration of triglyceride (TG) in the cells was measured by kit, and the lipid droplets was observed by oil red O staining. Immunoprecipitation and mass spectrometry were used to find the interesting proteins interacting with S100A16, and the interaction was verified by immunoprecipitation. The further mechanism was studied by Western blot and qRT-PCR. The results showed that the intracellular lipid droplet and TG concentrations in the fatty acid culture model were significantly higher than those in the control model. The accumulation of intracellular fat in the S100a16 over-expression group was significantly higher than that in the vector plasmid transfection group. There was an interaction between heat shock protein A5 (HSPA5) and S100A16. Over-expression of S100A16 up-regulated protein expression levels of HSPA5, inositol- requiring enzyme 1α (IRE1α) and pIREα1, which belong to endoplasmic reticulum stress HSPA5/IRE1α-XBP1 pathway. Meanwhile, over-expression of S100A16 up-regulated the mRNA expression levels of adipose synthesis-related gene Srebp1c, Acc and Fas. In the S100a16 shRNA plasmid transfection group, the above-mentioned protein and mRNA levels were lower than those of vector plasmid transfection group. These results suggest that S100A16 may promote lipid synthesis in HepG2 cells through endoplasmic reticulum stress HSPA5/IRE1α-XBP1 pathway.

Key words: S100A16; lipid metabolism; HSPA5; endoplasmic reticulum stress

Received: 2018-10-15　　Accepted: 2018-11-14

Corresponding author: 刘云　　E-mail: liuyun@njmu.edu.cn

DOI: 10.13294/j.aps.2019.0027

Citing This Article：

KAN Jing-Bao, SHEN Ge-Qian, YANG Jie, TONG Pei, ZHANG Ri-Hua, LIANG Xiu-Bin, SU Dong-Ming, LI Dong, LIU Yun. S100 calcium binding protein A16 promotes fat synthesis through endoplasmic reticulum stress in HepG2 cells . Acta Physiol Sin 2019; 71 (2): 279-286 (in Chinese with English abstract).