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Effects of FBI-1 silencing on proliferation and apoptosis of triple-negative breast cancer cell line MDA-MB-231

CHEN Mao-Jian¹, Wang Li², YANG Wei-Ping¹, QIN Qing-Hong¹, TAN Qi-Xing¹, LIAN Bin¹, WEI Chang-Yuan^1,*

¹Department of Breast Surgery, The Affiliated Tumor Hospital of Guangxi Medical University, Nanning 530021, China；²Department of Breast Surgery, Liuzhou People’s Hospital, Liuzhou 545006, China

Abstract

This work aimed to observe the effects of short hairpin RNA (shRNA)-silenced FBI-1 (factor that binds to the inducer of short transcripts of human immunodeficiency virus-1) on proliferation and apoptosis of triple-negative breast cancer cell line MDA-MB-231. qRT-PCR and Western blot analysis were applied to detect the mRNA and/or protein expression of FBI-1, Bcl-2, Bax, cleaved-Caspase 3 and Survivin. RNA interference method was used to silence FBI-1 expression in MDA-MB-231 cells. CCK-8 and colony formation assay were employed to detect the cell proliferation. Flow cytometry was employed for examining cell apoptosis. In vivo tumorigenicity of MDA-MB-231 cells was detected by tumor transplantation in nude mice. The results showed that the mRNA and protein expressions of FBI-1 were higher in MDA-MB-231 cells compared with those in normal human mammary epithelial cells MCF-10A. FBI-1 gene silencing inhibited proliferation and induced apoptosis of MDA-MB-231 cells in vitro, together with decreased Bcl-2 and Survivin protein expression, increased Bax protein expression and activated Caspase 3. Moreover, FBI-1 gene silencing 

inhibited the tumorigenesis of MDA-MB-231 cells in vivo. These results suggest that silencing of FBI-1 gene inhibits proliferation, induces apoptosis and suppresses the tumorigenesis of MDA-MB-231 cells.

Key words: FBI-1; short hairpin RNA; triple-negative breast cancer; cell proliferation; cell apoptosis

Received: 2018-02-01　　Accepted: 2018-08-14

Corresponding author: 韦长元　　E-mail: weicy63@aliyun.com

DOI: 10.13294/j.aps.2018.0056

Citing This Article：

CHEN Mao-Jian, Wang Li, YANG Wei-Ping, QIN Qing-Hong, TAN Qi-Xing, LIAN Bin, WEI Chang-Yuan. Effects of FBI-1 silencing on proliferation and apoptosis of triple-negative breast cancer cell line MDA-MB-231. Acta Physiol Sin 2018; 70 (5): 497-503 (in Chinese with English abstract).