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Differentially expressed circular RNAs in human gastric cancer cells

LONG Zhi¹, XIE Juan², LIU Yan-Ping¹, ZHANG Li¹, XU Chen-Guang¹, LUO Jiang-Yan¹, LI Guo-Qing^1,*

¹Department of Gastroenterology, the Second Affiliated Hospital of University of South China, Hengyang 421001, China；²Teaching and Research Section of Diagnosis, School of Medicine, University of South China, Hengyang 421001, China

Abstract

 The purpose of this study was to investigate the expression profile of circular RNA (circRNA) in gastric cancer cells MGC-803, SGC-7901 and NCI-N87 with different degrees of differentiation and normal gastric epithelial cells GES-1. High throughput circRNA microarray technique was used to detect the differential expression of circRNA between three kinds of differentiated gastric cancer cells and normal gastric epithelial cells. The interaction of microRNAs (miRNAs) with circRNAs was predicted by bioinformatics software, and circRNA, which might have great significance in gastric cancer, was identified by literature search. The results showed that there were 79 up-regulated circRNAs and 229 down-regulated circRNAs in gastric cancer cells with different degrees of differentiation compared with those in normal gastric epithelial cells. Through bioinformatics software analysis and literature retrieval, it was found that hsa_circ_0001897 was related to the staging and metastasis of gastric cancer, while the miR-150-5p, which combined with it, was closely related to many kinds of digestive tract tumors, such as colon cancer, liver cancer, and cholangiocarcinoma. The miR-16-5p, which has binding sites with hsa_circ_0008106 and hsa_circ_0060456, has been confirmed to be involved in the development of gastric cancer. The above results suggest that hsa_circ_0001897, hsa_circ_0008106 and hsa_circ_0060456 may be closely related to the occurrence and development of gastric cancer by interacting with miRNA.

Key words: circRNA; gastric cancer; microarray

Received: 2018-03-27　　Accepted: 2018-05-25

Corresponding author: 李国庆　　E-mail: ligq1970@163.com

DOI: 10.13294/j.aps.2018.0041

Citing This Article：

LONG Zhi, XIE Juan, LIU Yan-Ping, ZHANG Li, XU Chen-Guang, LUO Jiang-Yan, LI Guo-Qing. Differentially expressed circular RNAs in human gastric cancer cells. Acta Physiol Sin 2018; 70 (4): 384-390 (in Chinese with English abstract).