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Electrophysiological identification of human induced pluripotent stem cell- derived cardiomyocytes

ZHANG Hong-Yuan^1,2, ZHANG Ting-Ting^1,2, Machuki Jeremiah Ong’achwa¹, WU Li-Juan^1,2, Sun Hong^1,*

¹Department of Physiology, Xuzhou Medical University, Xuzhou 221004, China；²Institute of Cardiovascular Disease Research, Xuzhou Medical University, Xuzhou 221004, China

Abstract

The present study was aimed to characterize the electrophysiology of human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs). IMR90-4 cells were induced to differentiate into cardiomyocytes by temporal modulation of regulators of canonical Wnt signaling. The protein expression of cardiac troponin T (cTnT) was detected by immunofluorescence staining and flow cytometry, and the differentiation rate of hiPSC-CMs was calculated. The action potentials (APs) of hiPSC-CMs were recorded by patch clamp and used to classify different types of cardiomyocytes. The electrophysiological characteristics of hiPSC-CMs were further analyzed. The results showed that the cTnT positive rate of hiPSC-CMs was above 95%. hiPSC-CMs were differentiated into 3 types of cardiomyocytes based on the properties of AP: ventricular-, atrial- and nodal-like cells. In comparison with the other two types of cells, the APs of ventricular-like cells exhibited longer duration, higher amplitude and higher dV/dtmax. The nodal-like cells had the lowest dV/dtmax among all the three types. These results indicate that hiPSC can be differentiated into the cardiomyocytes with high purity and the differentiated hiPSC-CMs have similar electrophysiological characteristics to adult cardiomyocytes.

Key words: human induced pluripotent stem cell; cardiomyocyte; action potential; electrophysiology

Received: 2017-09-11　　Accepted: 2018-01-11

Corresponding author: 孙红　　E-mail: sunh@xzhmu.edu.cn

DOI: 10.13294/j.aps.2018.0040

Citing This Article：

ZHANG Hong-Yuan, ZHANG Ting-Ting, Machuki Jeremiah Ong’achwa, WU Li-Juan, Sun Hong. Electrophysiological identification of human induced pluripotent stem cell- derived cardiomyocytes. Acta Physiol Sin 2018; 70 (3): 281-286 (in Chinese with English abstract).