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Injury of human gastric epithelial GES-1 cells by MNNG and its effects on Wnt/β-catenin signaling pathway

YAN Zhan-Peng^1,2, XU Ting-Ting^1,2, AN Zhen-Tao^1,2, HU Ying^1,2, CHEN Wan-Zhen^1,2, ZHU Fang-Shi^1,2,*

¹Affiliated Hospital of Integrated Traditional Chinese and Western Medicine, Nanjing University of Chinese Medicine, Nanjing 210028, China；²Jiangsu Province Institute of Traditional Chinese Medicine, Nanjing 210028, China

Abstract

The aim of this study was to investigate the mechanisms of mono-functional alkylating agent MNNG to damage human gastric epithelial GES-1 cells and roles of Wnt/β-catenin signaling pathway in the process. The GES-1 cells were treated with MNNG (2 × 10−5 mol/L) for 24 h. The morphological changes of the GES-1 cells were observed under inverted microscope 2 d after treatment. The cell viability was measured by MTT assay. The apoptosis and cell cycle distribution of the GES-1 cells were analyzed by flow cytometry. The mRNA expressions of β-catenin, GSK-3β, c-Met and MMP7 in the GES-1 cells were detected by qPCR. The protein expressions of β-catenin, GSK-3β, p-GSK-3β and c-Met were determined by Western blot. The results showed that MNNG induced the injury of GES-1 cells and changed the normal cell morphology to irregular long spindle shape. MNNG induced the apoptosis of GES-1 cells and blocked the cell cycle progression obviously. MNNG up-regulated the mRNA expressions of β-catenin, GSK-3β, c-Met and MMP7, and increased the protein expressions of β-catenin, GSK-3β and p-GSK-3β. These results suggest that the damage of GES-1 cells induced by MNNG may be related to the activation of Wnt/β-catenin signaling pathway, which will provide the basis for the study of cell model of gastric mucosal cell injury.

Key words: Wnt/β-catenin signaling pathway; gastric mucosa epithelial cells; damage mechanisms

Received: 2017-11-17　　Accepted: 2018-01-04

Corresponding author: 朱方石　　E-mail: zhufangshi@126.com

DOI: 10.13294/j.aps.2018.0042

Citing This Article：

YAN Zhan-Peng, XU Ting-Ting, AN Zhen-Tao, HU Ying, CHEN Wan-Zhen, ZHU Fang-Shi. Injury of human gastric epithelial GES-1 cells by MNNG and its effects on Wnt/β-catenin signaling pathway. Acta Physiol Sin 2018; 70 (3): 262-268 (in Chinese with English abstract).