Issue Archive

Adenovirus-mediated overexpression of thioredoxin interaction protein inhibits INS-1 islet β cell proliferation

CAO Zhu-Jie, FENG Yan-Jin, LI Dan, Wang Jin, HUO Hai-Yan, ZHANG Xu-Mei, JIAO Xiang-Ying^*

partment of Physiology, Key Laboratory for Cellular Physiology of Ministry of Education, Shanxi Medical University, Taiyuan 030001, China

Abstract

Diabetes can cause a significant increase in the expression of thioredoxin (Trx)-interacting protein (TXNIP), which binds to Trx and inhibits its activity. The present study was aimed to investigate the effect of TXNIP on proliferation of rat INS-1 islet β cells and the underlying mechanism. TXNIP overexpressing adenovirus vectors (Ad-TXNIP-GFP and Ad-TXNIPc247s-GFP) were constructed and used to infect INS-1 cells. Ad-TXNIPc247s-GFP vector carries a mutant C247S TXNIP gene, and its expression product (TXNIPc247s) cannot attach and inhibit Trx activity. The expression of TXNIP was detected by real-time PCR and Western blot. EdU and Ki67 methods were used to detect cell proliferation. Protein phosphorylation levels of ERK and AKT were detected by Western blot. The results showed that both TXNIP and TXNIPc247s protein overexpressions inhibited the proliferation of INS-1 cells, and the former’s inhibitory effect was greater. Moreover, both of the two kinds of overexpressions inhibited the phosphorylation of ERK and AKT. These results suggest that TXNIP overexpression may inhibit the proliferation of INS-1 cells through Trx-dependent and non-Trx-dependent pathways, and the mechanism involves the inhibition of ERK and AKT phosphorylation.

Key words: adenovirus; thioredoxin-interacting protein; INS-1 cell; cell proliferation; ERK; Akt

Received: 2017-07-30　　Accepted: 2018-03-30

Corresponding author: 焦向英　　E-mail: jiaoxyty@163.com

DOI: 10.13294/j.aps.2018.0033

Citing This Article：

CAO Zhu-Jie, FENG Yan-Jin, LI Dan, Wang Jin, HUO Hai-Yan, ZHANG Xu-Mei, JIAO Xiang-Ying. Adenovirus-mediated overexpression of thioredoxin interaction protein inhibits INS-1 islet β cell proliferation. Acta Physiol Sin 2018; 70 (2): 158-166 (in Chinese with English abstract).