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Vasodilation of quercetin on rat renal artery and the relationship with L-type voltage-gated Ca²⁺ channels and protein kinase C

HOU Xiao-Min¹, ZHANG Ming-Sheng², QIN Xiao-Jiang^2,*

¹School of Basic Medical Sciences, Shanxi Medical University, Taiyuan 030001, China；²School of Public Health, Shanxi Medical University, Taiyuan 030001, China

Abstract

To investigate the diastolic function of quercetin on rat renal artery in vitro and its mechanism, the tension of rat renal artery was recorded by multi myograph system, and the L-type voltage-gated Ca²⁺ channels (LVGC) current was recorded by whole-cell patch clamp technique. Quercetin produced relaxation effect on rat renal artery pre-contracted by 60 mmol/L KCl or 1 × 10−5 mol/L phenylephrine, and the maximal diastolic percentage was  (84.53 ± 7.35)% or (76.42 ± 4.63)%. There was no statistical difference in the maximal diastolic percentage between endothelium-intact and endothelium-denuded groups. Pre-incubation of protein kinase C (PKC) inhibitor C6303 inhibited the maximal diastolic amplitude induced by quercetin. The peak current density of LVGC in rat renal artery vascular smooth muscle cells (VSMCs) was (23.17 ± 1.33) pA/pF. Quercetin (10 μmol/L) inhibited the peak current to (10.46 ± 1.35) pA/pF, and the inhibition percentage was 54.86%. C6303 (1 μmol/L) partially reversed the inhibitory effect of quercetin, and the inhibition percentage was 62.08% (P < 0.05). These results suggest that quercetin can relax rat renal artery in vitro in a concentration-dependent and endothelium- independent manner. The vasodilation of quercetin may be related to inhibition of LVGC current and activation of PKC.

Key words: quercetin; renal artery ; relaxation ; L-type voltage-gated Ca2+ channels ; protein kinase C

Received: 2017-05-03　　Accepted: 2017-10-13

Corresponding author: 秦小江　　E-mail: sxykdxyxy@163.com

DOI: 10.13294/j.aps.2017.0079

Citing This Article：

HOU Xiao-Min, ZHANG Ming-Sheng, QIN Xiao-Jiang. Vasodilation of quercetin on rat renal artery and the relationship with L-type voltage-gated Ca²⁺ channels and protein kinase C. Acta Physiol Sin 2017; 69 (6): 775-780 (in Chinese with English abstract).