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Effects of acute hypoxia on telomere length of rat gastric mucosa tissue and underlying mechanism

WANG Ya-Ping, ZHU Zhi-Yong, TANG Ying, MA Ying-Cai

Digestive Department of Qinghai Provincial People’s Hospital, Xining 810007, China

Abstract

The present study was aimed to investigate the effect of acute hypoxia on telomere length of rat gastric mucosa tissue and possible mechanism. Forty male Wistar rats were randomly divided into control group (resided in Lanzhou, 1 500 m) and experimental group (hypoxia chamber, 5 000 m). The experimental group was further divided into 3 subgroups and exposed to hypoxia for 1, 3, 7 d (n = 10), respectively. The morphological changes of the gastric mucosa tissue were observed by HE staining. By means of real-time PCR, ELISA and chemical immunofluorescence methods, the telomere length, the mRNA and protein levels of telomerase reverse transcriptase (TERT), hypoxia-inducible factor 1α (HIF-1α) and HIF-2α, and reactive oxygen species (ROS) level in gastric mucosa tissue were measured, respectively. The results showed that, with the extension of hypoxia-exposure time, the injury in gastric mucosa cells progressively became worse, and telomere length was increased gradually, along with intracellular ROS generation. The changes of TERT and HIF-1α expressions induced by acute hypoxia were in the same trend as that of telomere length. There were positive correlations between TERT mRNA expression and telomere length and between TERT and HIF-1α expressions, but not between TERT and HIF-2α mRNA expressions. These results suggest that under acute severe hypoxia environment, ROS could damage the gastric mucosa tissue cells, meanwhile the expressions of TERT and telomerase activity may be up-regulated by HIF-1α, which can elongate the telomere length and protect gastric mucosa tissue against fatal injury.

Key words: Hypoxia; telomere ; telomerase reverse transcriptase ; hypoxia-inducible factor ; reactive oxygen species

Received: 2017-03-25　　Accepted: 2017-05-08

Corresponding author: 王亚平　　E-mail: rose19820721@126.com

Citing This Article：

WANG Ya-Ping, ZHU Zhi-Yong, TANG Ying, MA Ying-Cai. Effects of acute hypoxia on telomere length of rat gastric mucosa tissue and underlying mechanism. Acta Physiol Sin 2017; 69 (4): 429-436 (in Chinese with English abstract).