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[Diverse components of voltage-gated calcium currents in primary cultured hippocampal neurons.] [Article in Chinese]

LIN Zhi-Ying, CHEN Xiao-Chun, ZHANG Jing, HUANG Tian-Wen

Department of Neurology； Fujian Institute of Geriatrics, the Affiliated Union Hospital of Fujian Medical University； Research Centre of Neurobiology, Fujian Medical University, Fuzhou 350001, China

Abstract

The present study was to record and separate the voltage-gated calcium channel currents (VGCCs) in primary cultured hippocampal neurons. An improved method is described for efficient and stable recording of VGCCs from primary cultured hippocampal neurons. The procedure allows the obtained hippocampal neurons overcome the defects from acutely isolated hippocampal neurons and currents do not rundown apparently after 20 min of membrane rupture. Using whole-cell configurations of patch clamp technique, it is shown that the method does not damage the neuron membrane electrical properties, and successfully records a variety of VGCCs components in hippocampal neurons (such as L, N, P/Q, T, etc.).

Key words: primary cultured; hippocampal neuron; voltage-gated calcium channel current; patch clamp techniques; high voltage activated calcium current

Received: 2012-04-28　　Accepted: 2012-09-06

Corresponding author: 陈晓春　　E-mail: chenxiaochun998@gmail.com

Citing This Article：

LIN Zhi-Ying, CHEN Xiao-Chun, ZHANG Jing, HUANG Tian-Wen. [Diverse components of voltage-gated calcium currents in primary cultured hippocampal neurons.] [Article in Chinese] . Acta Physiol Sin 2012; 64 (6): 687-694 (in Chinese with English abstract).