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[The antiepileptic role of 3α-androstanediol on the epileptic rats induced by pentylenetetrazole.] [Article in Chinese]

ZHANG YuHao, CAO YinXiang, MA Yu, GUO Xi, WANG Xin

Department of Neurology, Zhongshan Hospital, Fudan University, Shanghai 200032, China； Department of Physiology and Pathophysiology, Shanghai Medical College, Fudan University, Shanghai 200032, China； Department of Medicine, Branch of Zhongshan Hospital, Fudan University, Shanghai 200052, China； Institutes of Brain Science, State Key Laboratory of Medical Neurobiology, Fudan University, Shanghai 200032, China.

Abstract

The influence of 3α-androstanediol (3α-diol) on twitch and electroencephalogram (EEG) of the epileptic rats induced by pentylenetetrazole (PTZ) has been observed in this experiment in order to comprehensively explore the role of 3α-diol on epileptic attack from the aspects of behavior and EEG. Thirty-two male Sprague-Dawley rats were evenly and randomly divided into 4 groups: the normal and supplied with oil epileptic (N+oil+PTZ) group, the normal and supplied with 3α-diol epileptic (N+3α-diol+PTZ) group, the gonadectomized and supplied with oil epileptic (GDX+oil+PTZ) group and the gonadectomized and supplied with 3α-diol epileptic (GDX+3α-diol+PTZ) group. The changes of the behavior and EEG of epileptic rats in every group were recorded and analyzed. The results of behavior observation showed that the latency to clonic seizure and tonic-clonic seizure was shortened and the number of tonic-clonic seizure was increased significantly in the GDX+oil+PTZ group in comparison with N+oil+PTZ group (P < 0.05); comparing GDX+3α-diol+PTZ group with GDX+oil+PTZ group, or N+3α-diol+PTZ group with N+oil+PTZ group, we found that the latency to clonic seizure and tonic-clonic seizure became prolonged significantly, and the number of clonic seizure and tonic-clonic seizure was decreased significantly (P < 0.05). The results of EEG showed that the latency to epileptic waves was cut and the number of epileptic waves was augmented significantly in the GDX+oil+PTZ group in comparison with N+oil+PTZ group (P < 0.05); comparing GDX+3α-diol+PTZ group with GDX+oil+PTZ group, or N+3α-diol+PTZ group with N+oil+PTZ group, we found that the latency to epileptic waves became lengthened significantly, the number of epileptic waves was reduced significantly and the percentage of change of TP (total power of spectrum) was lessened significantly (P < 0.05). These results indicate that 3α-diol has an antiepileptic activity in the gonadectomized and normal epileptic rats.

Key words: Epilepsy; 3α-diol; behavior; EEG; pentylenetetrazole

Received: 2012-03-21　　Accepted: 2012-09-24

Corresponding author: 汪昕　　E-mail: wang.xin@zs-hospital.sh.cn

Citing This Article：

ZHANG YuHao, CAO YinXiang, MA Yu, GUO Xi, WANG Xin. [The antiepileptic role of 3α-androstanediol on the epileptic rats induced by pentylenetetrazole.] [Article in Chinese]. Acta Physiol Sin 2012; 64 (6): 633-638 (in Chinese with English abstract).