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[Effects of linoleic acid on intracellular calcium concentration in primarily cultured rat pancreatic β -cells and underlying mechanism.] [Ariticle in Chinese]

WANG Li, FU Rong-Guo, LIU Xiao-Dan, GUI Bao Song, SUN Qiang, CHEN Chen, ZHAO Yu-Feng, DONG Lei

The Second Affiliated Hospital of Medical School, Xi’an Jiao Tong University, Xi’an 710004, China； Medical School of Xi’an Jiao Tong University, Xi’an 710061, China； Experiment Teaching Center, Fourth Military Medical University, Xi’an 710032, China

Abstract

In this study, we investigated the mechanism of linoleic acid-stimulated increase in intracellular calcium concentration ([Ca2+]i) in pancreatic islet β-cells. Pancreatic islet cells were primarily isolated from rats and cultured for the experiments. The cells were loaded with Fluo-3/AM, the indicator of [Ca2+]i, and the intensity of Fluo-3 was measured using confocal microscope. The islet β-cells were identified by immunocytochemical staining with insulin antibody after recording. The drugs were given by perfusion system. The results showed that linoleic acid (20 μmol/L) stimulated [Ca2+]i increase with the first peak increase and the following plateau increase. Linoleic acid-stimulated [Ca2+]i increase was partly inhibited by removal of extracellular calcium and by transient receptor potential (TRP) channel blocker, La3+, and it was totally blocked by exhaustion of intracellular calcium stores and inhibition of phospholipase C. It is concluded that linoleic acid stimulates [Ca2+]i increase in islet β-cells through both extracellular calcium influx via TRP channels and calcium release from intracellular calcium stores.

Key words: linoleic acid; islets; β-cells

Received: 2010-09-24　　Accepted: 2010-11-08

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Citing This Article：

WANG Li, FU Rong-Guo, LIU Xiao-Dan, GUI Bao Song, SUN Qiang, CHEN Chen, ZHAO Yu-Feng, DONG Lei. [Effects of linoleic acid on intracellular calcium concentration in primarily cultured rat pancreatic β -cells and underlying mechanism.] [Ariticle in Chinese] . Acta Physiol Sin 2010; 62 (6): 529-534 (in Chinese with English abstract).